ArticleThe Journal of biological chemistry2009
Molecular recognition of the palmitoylation substrate Vac8 by its palmitoyltransferase Pfa3.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 52 citations in OpenAlex.
- Palmitoylation of ULK1 by ZDHHC13 plays a crucial role in autophagy.Nature communications · 2024Article
- Regulation of Dynamic Protein S-Acylation.Frontiers in molecular biosciences · 2021Review
- Structure and Mechanism of DHHC Protein Acyltransferases.Journal of molecular biology · 2020Review
- Article
- Spatial organization of palmitoyl acyl transferases governs substrate localization and function.Molecular membrane biology · 2019Review
- Article
- Multilayered regulation of TORC1-body formation in budding yeast.Molecular biology of the cell · 2019Article
- Regulation of Sensing, Transportation, and Catabolism of Nitrogen Sources in Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 2018Review
- The Architecture of the Rag GTPase Signaling Network.Biomolecules · 2017Review
- An amphipathic α-helix directs palmitoylation of the large intracellular loop of the sodium/calcium exchanger.The Journal of biological chemistry · 2017Article
- Unsolved mysteries of Rag GTPase signaling in yeast.Small GTPases · 2016Article
- Conserved regulators of Rag GTPases orchestrate amino acid-dependent TORC1 signaling.Cell discovery · 2016Review
- The physiology of protein S-acylation.Physiological reviews · 2015Review
- Substrate recognition by the cell surface palmitoyl transferase DHHC5.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Distinct palmitoylation events at the amino-terminal conserved cysteines of Env7 direct its stability, localization, and vacuolar fusion regulation in S. cerevisiae.The Journal of biological chemistry · 2014Article
- Quantitative control of protein S-palmitoylation regulates meiotic entry in fission yeast.PLoS biology · 2013Article
- Saccharomyces cerevisiae Env7 is a novel serine/threonine kinase 16-related protein kinase and negatively regulates organelle fusion at the lysosomal vacuole.Molecular and cellular biology · 2013Article
- Palmitoylation of virus proteins.Biology of the cell · 2012Review
- S-acylation-dependent association of the calcium sensor CBL2 with the vacuolar membrane is essential for proper abscisic acid responses.Cell research · 2012Article
- An electrostatic switch controls palmitoylation of the large conductance voltage- and calcium-activated potassium (BK) channel.The Journal of biological chemistry · 2012Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Palmitoylation of the yeast vacuolar protein Vac8 is important for its role in membrane-mediated events such as vacuole fusion. It has been established both in vivo and in vitro that Vac8 is palmitoylated by the Asp-His-His-Cys (DHHC) protein Pfa3. However, the determinants of Vac8 critical for recognition by Pfa3 have yet to be elucidated. This is of particular importance because of the lack of a consensus sequence for palmitoylation. Here we show that Pfa3 was capable of palmitoylating each of the three N-terminal cysteines of Vac8 and that this reaction was most efficient when Vac8 is N-myristoylated. Additionally, when we analyzed the Src homology 4 (SH4) domain of Vac8 independent of the rest of the protein, palmitoylation by Pfa3 still occurred. However, the specificity of palmitoylation seen for the full-length protein was lost, and the SH4 domain was palmitoylated by all five of the yeast DHHC proteins tested. These data suggested that a region of the protein C-terminal to the SH4 domain was important for conferring specificity of palmitoylation. This was confirmed by use of a chimeric protein in which the SH4 domain of Vac8 was swapped for that of Meh1, another palmitoylated and N-myristoylated protein in yeast. In this case we saw specificity mimic that of wild type Vac8. Competition experiments revealed that the 11th armadillo repeat of Vac8 is an important element for recognition by Pfa3. This demonstrates that regions distant from the palmitoylated cysteines are important for recognition by DHHC proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.