ArticleProceedings of the National Academy of Sciences of the United States of America2009
Inactivation of expression of several genes in a variety of bacterial species by EGS technology.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Restoring susceptibility to aminoglycosides: identifying small molecule inhibitors of enzymatic inactivation.RSC medicinal chemistry · 2023Review
- Global RNA profiles show target selectivity and physiological effects of peptide-delivered antisense antibiotics.Nucleic acids research · 2021Article
- Silencing Antibiotic Resistance with Antisense Oligonucleotides.Biomedicines · 2021Review
- Advances in therapeutic bacterial antisense biotechnology.Applied microbiology and biotechnology · 2018Review
- Identification of theFrontiers in microbiology · 2018Article
- SalmoNet, an integrated network of tenNPJ systems biology and applications · 2017Article
- Targeting the Bacterial Division Protein FtsZ.Journal of medicinal chemistry · 2016Review
- Assessment of configurations and chemistries of bridged nucleic acids-containing oligomers as external guide sequences: a methodology for inhibition of expression of antibiotic resistance genes.Biology methods & protocols · 2016Article
- RNase P-Mediated Sequence-Specific Cleavage of RNA by Engineered External Guide Sequences.Biomolecules · 2015Review
- External guide sequence technology: a path to development of novel antimicrobial therapeutics.Annals of the New York Academy of Sciences · 2015Review
- Article
- A peptide-morpholino oligomer conjugate targeting Staphylococcus aureus gyrA mRNA improves healing in an infected mouse cutaneous wound model.International journal of pharmaceutics · 2013Article
- Microbial competition in polar soils: a review of an understudied but potentially important control on productivity.Biology · 2013Article
- Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2).PloS one · 2013Article
- Internalization of Locked Nucleic Acids/DNA Hybrid Oligomers into Escherichia coli.BioResearch open access · 2012Article
- Gene selective mRNA cleavage inhibits the development of Plasmodium falciparum.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- RNA therapeutics: beyond RNA interference and antisense oligonucleotides.Nature reviews. Drug discovery · 2012Review
- Inhibition of cell division induced by external guide sequences (EGS Technology) targeting ftsZ.PloS one · 2012Article
- Ribonuclease P.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2011Article
- Basic peptide-morpholino oligomer conjugate that is very effective in killing bacteria by gene-specific and nonspecific modes.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expression of gene products in bacteria can be inhibited by the use of RNA external guide sequences (EGSs) that hybridize to a target mRNA. Endogenous RNase P cleaves the mRNA in the complex, making it inactive. EGSs participate in this biochemical reaction as the data presented here show. They promote mRNA cleavage at the expected site and sometimes at other secondary sites. Higher-order structure must affect these reactions if the cleavage does not occur at the defined site, which has been determined by techniques based on their ability to find sites that are accessible to the EGS oligonucleotides. Sites defined by a random EGS technique occur as expected. Oligonucleotides made up primarily of defined or random nucleotides are extremely useful in inhibiting expression of the gyrA and rnpA genes from several different bacteria or the cat gene that determines resistance to chloramphenicol in Escherichia coli. An EGS made up of a peptide-phosphorodiamidate morpholino oligonucleotide (PPMO) does not cleave at the same site as an unmodified RNA EGS for reasons that are only partly understood. However, PPMO-EGSs are useful in inhibiting the expression of targeted genes from Gram-negative and Gram-positive organisms during ordinary growth in broth and may provide a basis for broad-spectrum antibiotics.
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