ArticleBMC biology2009
F-actin-based extensions of the head cyst cell adhere to the maturing spermatids to maintain them in a tight bundle and prevent their premature release in Drosophila testis.
Article in BMC biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 43 citations in OpenAlex.
- Togaram Ensures Axial Alignment of the Sperm Neck.bioRxiv : the preprint server for biology · 2026Article
- Soma-to-germline BMP signal is essential for Drosophila spermiogenesis.Developmental biology · 2025Article
- Reproductive Toxicity of Nanomaterials Using Silver Nanoparticles andMolecules (Basel, Switzerland) · 2024Article
- The evolutionarily conserved PhLP3 is essential for sperm development in Drosophila melanogaster.PloS one · 2024Article
- Interlocking of co-opted developmental gene networks in Drosophila and the evolution of pre-adaptive novelty.Nature communications · 2023Article
- Article
- Gap junctions mediate discrete regulatory steps during fly spermatogenesis.PLoS genetics · 2022Article
- Time-lapse imaging ofSTAR protocols · 2022Article
- Long noncoding RNA regulation of spermatogenesis via the spectrin cytoskeleton in Drosophila.G3 (Bethesda, Md.) · 2021Article
- A conditional Orco requirement in the somatic cyst cells for maintaining spermatids in a tight bundle in Drosophila testis.Journal of biosciences · 2016Article
- Article
- The Drosophila cyst stem cell lineage: Partners behind the scenes?Spermatogenesis · 2012Article
- Blanks, a nuclear siRNA/dsRNA-binding complex component, is required for Drosophila spermiogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Article
- Spermiation: The process of sperm release.Spermatogenesis · 2011Article
- Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn Drosophila, all the 64 clonally derived spermatocytes differentiate in syncytium inside two somatic-origin cyst cells. They elongate to form slender spermatids, which are individualized and then released into the seminal vesicle. During individualization, differentiating spermatids are organized in a tight bundle inside the cyst, which is expected to play an important role in sperm selection. However, actual significance of this process and its underlying mechanism are unclear.
resultsWe show that dynamic F-actin-based processes extend from the head cyst cell at the start of individualization, filling the interstitial space at the rostral ends of the maturing spermatid bundle. In addition to actin, these structures contained lamin, beta-catenin, dynamin, myosin VI and several other filopodial components. Further, pharmacological and genetic analyses showed that cytoskeletal stability and dynamin function are essential for their maintenance. Disruption of these F-actin based processes was associated with spermatid bundle disassembly and premature sperm release inside the testis.
conclusionAltogether, our data suggests that the head cyst cell adheres to the maturing spermatid heads through F-actin-based extensions, thus maintaining them in a tight bundle. This is likely to regulate mature sperm release into the seminal vesicle. Overall, this process bears resemblance to mammalian spermiation.
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