ArticleGenesis (New York, N.Y. : 2000)2009
FlEx-based transgenic reporter lines for visualization of Cre and Flp activity in live zebrafish.
Article in Genesis (New York, N.Y. : 2000), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
50 citing papers in PubMed, 84 citations in OpenAlex.
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- Non-apoptotic caspase events and Atf3 expression underlie direct neuronal differentiation of adult neural stem cells.Development (Cambridge, England) · 2024Article
- Reconstruction of macroglia and adult neurogenesis evolution through cross-species single-cell transcriptomic analyses.Nature communications · 2024Article
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- Highly selective transgene expression through the flip-excision switch system by using a unilateral spacer sequence.Cell reports methods · 2023Article
- Small fish, big discoveries: zebrafish shed light on microbial biomarkers for neuro-immune-cardiovascular health.Frontiers in physiology · 2023Review
- zMADM (zebrafish mosaic analysis with double markers) for single-cell gene knockout and dual-lineage tracing.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- longfin causes cis-ectopic expression of the kcnh2a ether-a-go-go K+ channel to autonomously prolong fin outgrowth.Development (Cambridge, England) · 2021Article
- PINK1 deficiency impairs adult neurogenesis of dopaminergic neurons.Scientific reports · 2021Article
- Genetic Approaches Using Zebrafish to Study the Microbiota-Gut-Brain Axis in Neurological Disorders.Cells · 2021Review
- Taste buds are not derived from neural crest in mouse, chicken, and zebrafish.Developmental biology · 2021Article
- Lineage hierarchies and stochasticity ensure the long-term maintenance of adult neural stem cells.Science advances · 2020Article
- Regeneration of Dopaminergic Neurons in Adult Zebrafish Depends on Immune System Activation and Differs for Distinct Populations.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Analysis of a conditional gene trap reveals that tbx5a is required for heart regeneration in zebrafish.PloS one · 2018Article
- Life-Long Neurogenic Activity of Individual Neural Stem Cells and Continuous Growth Establish an Outside-In Architecture in the Teleost Pallium.Current biology : CB · 2017Article
- Genetic targeting and anatomical registration of neuronal populations in the zebrafish brain with a new set of BAC transgenic tools.Scientific reports · 2017Article
- Embryonic origin and lineage hierarchies of the neural progenitor subtypes building the zebrafish adult midbrain.Developmental biology · 2016Article
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- Long-termLight, science & applications · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Site-specific recombinases such as Cre and Flp are invaluable tools for genetic manipulations, but their usage in zebrafish has been limited. Incorporating recently developed flip-excision (FlEx) design that allows stable inversions, we have established zebrafish reporter lines that express bright and ubiquitous EGFP, but switch to express mCherry in the presence of Cre or Flp. Here, we demonstrate the stable inversion in the reporter lines, both in somatic cells and in the germ line by Cre or Flp, and the subsequent reinversion using the other recombinase. Using the reporter lines, we characterized cardiomyocyte-specific Cre lines and neuronal progenitor-specific and tamoxifen-dependent Cre lines. We also used the reporter lines for screening Cre- and Flp-based enhancer trap lines. Similar to the widely used Cre reporter lines in mice, these FlEx-based reporter lines will facilitate the use of recombinases for genetic manipulations in zebrafish.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.