ArticleJournal of molecular biology2009
Structural and functional characterisation of a conserved archaeal RadA paralog with antirecombinase activity.
Article in Journal of molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- The Impact of Single-Stranded DNA-Binding Protein SSB and Putative SSB-Interacting Proteins on Genome Integrity in the Thermophilic CrenarchaeonInternational journal of molecular sciences · 2023Article
- Take a Break to Repair: A Dip in the World of Double-Strand Break Repair Mechanisms Pointing the Gaze on Archaea.International journal of molecular sciences · 2021Review
- Role of RadA and DNA Polymerases in Recombination-Associated DNA Synthesis in Hyperthermophilic Archaea.Biomolecules · 2020Article
- Phosphorylation of the Archaeal Holliday Junction Resolvase Hjc Inhibits Its Catalytic Activity and Facilitates DNA Repair inFrontiers in microbiology · 2019Article
- Sak4 of Phage HK620 Is a RecA Remote Homolog With Single-Strand Annealing Activity Stimulated by Its Cognate SSB Protein.Frontiers in microbiology · 2018Article
- Article
- Extreme Deviations from Expected Evolutionary Rates in Archaeal Protein Families.Genome biology and evolution · 2017Article
- Crystal structure of the flagellar accessory protein FlaH of Methanocaldococcus jannaschii suggests a regulatory role in archaeal flagellum assembly.Protein science : a publication of the Protein Society · 2016Article
- Post-translational environmental switch of RadA activity by extein-intein interactions in protein splicing.Nucleic acids research · 2015Article
- Mediators of homologous DNA pairing.Cold Spring Harbor perspectives in biology · 2014Review
- Nanobiomotors of archaeal DNA repair machineries: current research status and application potential.Cell & bioscience · 2014Review
- An archaeal RadA paralog influences presynaptic filament formation.DNA repair · 2013Article
- Article
- Experimental phasing using zinc anomalous scattering.Acta crystallographica. Section D, Biological crystallography · 2012Article
- Repair of DNA double-strand breaks following UV damage in three Sulfolobus solfataricus strains.Journal of bacteriology · 2010Article
- Insights into the mechanism of Rad51 recombinase from the structure and properties of a filament interface mutant.Nucleic acids research · 2010Article
- The Scottish Structural Proteomics Facility: targets, methods and outputs.Journal of structural and functional genomics · 2010Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
DNA recombinases (RecA in bacteria, Rad51 in eukarya and RadA in archaea) catalyse strand exchange between homologous DNA molecules, the central reaction of homologous recombination, and are among the most conserved DNA repair proteins known. RecA is the sole protein responsible for this reaction in bacteria, whereas there are several Rad51 paralogs that cooperate to catalyse strand exchange in eukaryotes. All archaea have at least one (and as many as four) RadA paralog, but their function remains unclear. Herein, we show that the three RadA paralogs encoded by the Sulfolobus solfataricus genome are expressed under normal growth conditions and are not UV inducible. We demonstrate that one of these proteins, Sso2452, which is representative of the large archaeal RadC subfamily of archaeal RadA paralogs, functions as an ATPase that binds tightly to single-stranded DNA. However, Sso2452 is not an active recombinase in vitro and inhibits D-loop formation by RadA. We present the high-resolution crystal structure of Sso2452, which reveals key structural differences from the canonical RecA family recombinases that may explain its functional properties. The possible roles of the archaeal RadA paralogs in vivo are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.