ArticleBiophysical journal2009
The intracellular mobility of nuclear import receptors and NLS cargoes.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Article
- Programmable gene insertion in human cells with a laboratory-evolved CRISPR-associated transposase.Science (New York, N.Y.) · 2025Article
- More than double the fun with two-photon excitation microscopy.Communications biology · 2024Review
- Article
- Review
- Karyopherins in the Remodeling of Extracellular Matrix: Implications in Tendon Injury.Journal of orthopaedics and sports medicine · 2023Article
- Prime Editing: An All-Rounder for Genome Editing.International journal of molecular sciences · 2022Review
- Enhanced prime editing systems by manipulating cellular determinants of editing outcomes.Cell · 2021Article
- Physical modeling of multivalent interactions in the nuclear pore complex.Biophysical journal · 2021Article
- MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo.Nucleic acids research · 2021Article
- YAP1/TAZ-TEAD transcriptional networks maintain skin homeostasis by regulating cell proliferation and limiting KLF4 activity.Nature communications · 2020Article
- CRISPR-Cas3 induces broad and unidirectional genome editing in human cells.Nature communications · 2019Article
- Improving Editing Efficiency for the Sequences with NGH PAM Using xCas9-Derived Base Editors.Molecular therapy. Nucleic acids · 2019Article
- A split CRISPR-Cpf1 platform for inducible genome editing and gene activation.Nature chemical biology · 2019Article
- Local, transient tensile stress on the nuclear membrane causes membrane rupture.Molecular biology of the cell · 2019Article
- Viral highway to nucleus exposed by image correlation analyses.Scientific reports · 2018Article
- In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration.Nature · 2016Article
- Association of Endophilin B1 with Cytoplasmic Vesicles.Biophysical journal · 2016Article
- Novel localization of formin mDia2: importin β-mediated delivery to and retention at the cytoplasmic side of the nuclear envelope.Biology open · 2015Article
- Yeast importin-α (Srp1) performs distinct roles in the import of nuclear proteins and in targeting proteasomes to the nucleus.The Journal of biological chemistry · 2014Article
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Authors and funding
3 authors.
Funding
Abstract
We have investigated classical nuclear localization sequence (NLS) mediated protein trafficking by measuring biomolecular dynamics within living cells using two-photon fluorescence correlation spectroscopy. By directly observing the behavior of specific molecules in their native cellular environment, it is possible to uncover functional details that are not apparent from traditional biochemical investigations or functional assays. We show that the intracellular mobility of NLS cargoes and their import receptor proteins, karyopherin-alpha and karyopherin-beta, can be robustly measured and that quantitative comparison of intracellular diffusion coefficients provides new insights into nuclear transport mechanisms. Import cargo complexes are assembled throughout the cytoplasm, and their diffusion is slower than predicted by molecular weight due to specific interactions. Analysis of NLS cargo diffusion in the cytoplasm indicates that these interactions are likely disrupted by NLS cargo binding. Our results suggest that delivery of import receptors and NLS cargoes to nuclear pores may complement selective translocation through the pores as a functional mechanism for regulating transport of proteins into the nucleus.
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