ArticleBiophysical journal2009
Reaction dynamics of halorhodopsin studied by time-resolved diffusion.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 17 citations in OpenAlex.
- Direct detection of the chloride release and uptake reactions of Natronomonas pharaonis halorhodopsin.The Journal of biological chemistry · 2024Article
- Functional Mechanism of ClAdvances in experimental medicine and biology · 2021Review
- Crystal structures of the L1, L2, N, and O states of pharaonis halorhodopsin.Biophysical journal · 2015Article
- Homotrimer formation and dissociation of pharaonis halorhodopsin in detergent system.Biophysical journal · 2012Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reaction dynamics of a chloride ion pump protein, halorhodopsin (HR), from Natronomonas pharaonis (N. pharaonis) (NpHR) was studied by the pulsed-laser-induced transient grating (TG) method. A detailed investigation of the TG signal revealed that there is a spectrally silent diffusion process besides the absorption-observable reaction dynamics. We interpreted these dynamics in terms of release, diffusion, and uptake of the Cl(-) ion. From a quantitative global analysis of the signals at various grating wavenumbers, it was concluded that the release of the Cl(-) ion is associated with the L2 --> (L2 (or N) <==> O) process, and uptake of Cl(-) occurs with the (L2 (or N) <==> O) -->NpHR' process. The diffusion coefficient of NpHR solubilized in a detergent did not change during the cyclic reaction. This result contrasts the behavior of many photosensor proteins and implies that the change in the H-bond network from intra- to intermolecular is not significant for the activity of this protein pump.
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Registered trials
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