ArticleJournal of cellular and molecular medicine
The alpha1H Ca2+ channel subunit is expressed in mouse jejunal interstitial cells of Cajal and myocytes.
Article in Journal of cellular and molecular medicine. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 36 citations in OpenAlex.
- A specialized population of intramuscular interstitial cells of Cajal regulates motility within the mouse oesophagogastric junction.The Journal of physiology · 2025Article
- Overview of Lymphatic Muscle Cells in Development, Physiology, and Disease.Microcirculation (New York, N.Y. : 1994) · 2024Review
- CaPhysiological reviews · 2024Review
- Transcriptome and Proteome Profiling of Primary Human Gastric Interstitial Cells of Cajal Predicts Pacemaker Networks.Journal of neurogastroenterology and motility · 2023Article
- Hyperpolarization-activated cyclic nucleotide-gated channels working as pacemaker channels in colonic interstitial cells of Cajal.Journal of cellular and molecular medicine · 2022Article
- CaAdvances in experimental medicine and biology · 2022Article
- Bicarbonate ion transport by the electrogenic NaNeurogastroenterology and motility · 2021Article
- Arecoline hydrobromide enhances jejunum smooth muscle contractility via voltage-dependent potassium channels in W/Wv mice.Physiological research · 2021Article
- Mechanotransduction in gastrointestinal smooth muscle cells: role of mechanosensitive ion channels.American journal of physiology. Gastrointestinal and liver physiology · 2021Review
- Expression of the regulated isoform of the electrogenic NaAmerican journal of physiology. Gastrointestinal and liver physiology · 2021Article
- Rhythmic calcium transients in smooth muscle cells of the mouse internal anal sphincter.Neurogastroenterology and motility · 2020Article
- Spontaneous Electrical Activity and Rhythmicity in Gastrointestinal Smooth Muscles.Advances in experimental medicine and biology · 2019Review
- Clustering of CaThe Journal of general physiology · 2017Article
- Changes in nitrergic and tachykininergic pathways in rat proximal colon in response to chronic treatment with otilonium bromide.Neurogastroenterology and motility · 2015Article
- Real-time measurement of biomagnetic vector fields in functional syncytium using amorphous metal.Scientific reports · 2015Article
- Characterization of slow waves generated by myenteric interstitial cells of Cajal of the rabbit small intestine.American journal of physiology. Gastrointestinal and liver physiology · 2015Article
- Colonic smooth muscle cells and colonic motility patterns as a target for irritable bowel syndrome therapy: mechanisms of action of otilonium bromide.Therapeutic advances in gastroenterology · 2014Review
- Interstitial cells: regulators of smooth muscle function.Physiological reviews · 2014Review
- Computational modeling of anoctamin 1 calcium-activated chloride channels as pacemaker channels in interstitial cells of Cajal.American journal of physiology. Gastrointestinal and liver physiology · 2014Article
- Expression and function of a T-type Ca2+ conductance in interstitial cells of Cajal of the murine small intestine.American journal of physiology. Cell physiology · 2014Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
T-type Ca(2+) currents have been detected in cells from the external muscular layers of gastrointestinal smooth muscles and appear to contribute to the generation of pacemaker potentials in interstitial cells of Cajal from those tissues. However, the Ca(2+) channel alpha subunit responsible for these currents has not been determined. We established that the alpha subunit of the alpha(1H) Ca(2+) channel is expressed in single myocytes and interstitial cells of Cajal using reverse transcription and polymerase chain reaction from whole tissue, laser capture microdissected tissue and single cells isolated from the mouse jejunum. Whole-cell voltage clamp recordings demonstrated that a nifedipine and Cd(2+) resistant, mibefradil-sensitive current is present in myocytes dissociated from the jejunum. Electrical recordings from the circular muscle layer demonstrated that mibefradil reduced the frequency and initial rate of rise of the electrical slow wave. Gene targeted knockout of both alleles of the cacna1h gene, which encodes the alpha(1H) Ca(2+) channel subunit, resulted in embryonic lethality because of death of the homozygous knockouts prior to E13.5 days in utero. We conclude that a channel with the pharmacological and molecular characteristics of the alpha(1H) Ca(2+) channel subunit is expressed in interstitial cells of Cajal and myocytes from the mouse jejunum, and that ionic conductances through the alpha(1H) Ca(2+) channel contribute to the upstroke of the pacemaker potential. Furthermore, the survival of mice that do not express the alpha(1H) Ca(2+) channel protein is dependent on the genetic background and targeting approach used to generate the knockout mice.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.