ArticleBiochemistry2009
Effects of recombinant protein expression on green fluorescent protein diffusion in Escherichia coli.
Article in Biochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 51 citations in OpenAlex.
- PySupercharge: a python algorithm for enabling ABC transporter bacterial secretion of all proteins through amino acid mutation.Microbial cell factories · 2024Article
- Controlling and quantifying protein concentration in Escherichia coli.Protein science : a publication of the Protein Society · 2019Article
- Resolving Cytosolic Diffusive States in Bacteria by Single-Molecule Tracking.Biophysical journal · 2019Article
- In-cell NMR characterization of the secondary structure populations of a disordered conformation of α-synuclein within E. coli cells.PloS one · 2013Article
- Disordered Protein Diffusion under Crowded Conditions.The journal of physical chemistry letters · 2012Article
- In-cell NMR spectroscopy.Progress in nuclear magnetic resonance spectroscopy · 2011Review
- In-cell protein NMR and protein leakage.Proteins · 2011Article
- Evaluation of pulsed-FRAP and conventional-FRAP for determination of protein mobility in prokaryotic cells.PloS one · 2011Article
- Size dependence of protein diffusion in the cytoplasm of Escherichia coli.Journal of bacteriology · 2010Article
- A bioreactor for in-cell protein NMR.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2010Article
- Protein (19)F NMR in Escherichia coli.Journal of the American Chemical Society · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Fluorescence recovery after photobleaching was used to measure the diffusion coefficient of green fluorescent protein (GFP, 27 kDa) in Escherichia coli in the presence or absence of four coexpressed proteins: cytoplasmic maltose binding protein (42 kDa), tau-40 (45 kDa), alpha-synuclein (14 kDa), or calmodulin (17 kDa). The GFP diffusion coefficient remains constant regardless of the type of coexpresseed protein and whether or not the coexpressed protein was induced. We conclude that expression of these soluble proteins has little to no effect on the diffusion of GFP. These results have implications for the utility of in-cell nuclear magnetic resonance spectroscopy.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.