ArticleRapid communications in mass spectrometry : RCM2009
Synthesis of biotin-tagged chemical cross-linkers and their applications for mass spectrometry.
Article in Rapid communications in mass spectrometry : RCM, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Directed evolution of APOX for proximity labeling using phenols with high redox potentials.Cell chemical biology · 2026Article
- Higher-Order Structural Organization of the Mitochondrial Proteome Charted by In Situ Cross-Linking Mass Spectrometry.Molecular & cellular proteomics : MCP · 2023Article
- Cross-linking mass spectrometry for mapping protein complex topologies in situ.Essays in biochemistry · 2023Article
- An optimized protocol forSTAR protocols · 2022Article
- New insights into the mechanisms of age-related protein-protein crosslinking in the human lens.Experimental eye research · 2021Review
- MS-Based Approaches Enable the Structural Characterization of Transcription Factor/DNA Response Element Complex.Biomolecules · 2019Article
- PhoX: An IMAC-Enrichable Cross-Linking Reagent.ACS central science · 2019Article
- Protein Dynamics in Solution by Quantitative Crosslinking/Mass Spectrometry.Trends in biochemical sciences · 2018Review
- Synthesis and Avidin Binding of Ruthenium Complexes Functionalized with a Light-Cleavable Free Biotin Moiety.European journal of inorganic chemistry · 2018Article
- Bifunctional cross-linking approaches for mass spectrometry-based investigation of nucleic acids and protein-nucleic acid assemblies.Methods (San Diego, Calif.) · 2018Article
- Development of Large-scale Cross-linking Mass Spectrometry.Molecular & cellular proteomics : MCP · 2018Article
- Characterization of homodimer interfaces with cross-linking mass spectrometry and isotopically labeled proteins.Nature protocols · 2018Article
- Article
- The Use of Advanced Mass Spectrometry to Dissect the Life-Cycle of Photosystem II.Frontiers in plant science · 2016Review
- Kojak: efficient analysis of chemically cross-linked protein complexes.Journal of proteome research · 2015Article
- Enhanced identification of zero-length chemical cross-links using label-free quantitation and high-resolution fragment ion spectra.Journal of proteome research · 2014Article
- Designer reagents for mass spectrometry-based proteomics: clickable cross-linkers for elucidation of protein structures and interactions.Analytical chemistry · 2012Article
- Expanding the chemical cross-linking toolbox by the use of multiple proteases and enrichment by size exclusion chromatography.Molecular & cellular proteomics : MCP · 2012Article
- Quaternary diamines as mass spectrometry cleavable crosslinkers for protein interactions.Journal of the American Society for Mass Spectrometry · 2012Article
- Modern biomolecular mass spectrometry and its role in studying virus structure, dynamics, and assembly.Angewandte Chemie (International ed. in English) · 2011Review
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6 authors.
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Abstract
Chemical cross-linking combined with mass spectrometry (MS) has been used to elucidate protein structures and protein-protein interactions. However, heterogeneity of the samples and the relatively low abundance of cross-linked peptides make this approach challenging. As an effort to overcome this hurdle, we have synthesized lysine-reactive homobifunctional cross-linkers with the biotin in the middle of the linker and used them to enrich cross-linked peptides. The reaction of biotin-tagged cross-linkers with purified HIV-1 CA resulted in the formation of hanging and intramolecular cross-links. The peptides modified with biotinylated cross-linkers were effectively enriched and recovered using a streptavidin-coated plate and MS-friendly buffers. The enrichment of modified peptides and removal of the dominantly unmodified peptides simplify mass spectra and their analyses. The combination of the high mass accuracy of Fourier transform ion cyclotron resonance (FT-ICR) MS and the tandem mass spectrometric (MS/MS) capability of the linear ion trap allows us to unambiguously identify the cross-linking sites and additional modification, such as oxidation.
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