ArticleGenome research2009
Proteomic discovery of previously unannotated, rapidly evolving seminal fluid genes in Drosophila.
Article in Genome research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers.
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Who cites it
88 citing papers in PubMed.
- Abdominal-B regulates the male seminal fluid transferome and new fecundity factors required for sperm sex peptide binding.PLoS genetics · 2026Article
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- Evolutionary Analysis of Gene-Expression Localization in the Model Crustacean, Daphnia pulex.Molecular ecology · 2025Article
- Gene age shapes functional and evolutionary properties of theProceedings of the National Academy of Sciences of the United States of America · 2025Article
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- Evolutionary Analysis of Gene-expression Localization in the Model Crustacean,bioRxiv : the preprint server for biology · 2025Article
- An orphan gene is essential for efficient sperm entry into eggs in Drosophila melanogaster.Genetics · 2025Article
- Evolutionary innovations in germline biology of placental mammals identified by transcriptomics of first-wave spermatogenesis in opossum.Developmental cell · 2025Article
- An orphan gene is essential for efficient sperm entry into eggs inbioRxiv : the preprint server for biology · 2024Article
- Exposure to Titanium Dioxide Nanoparticles Leads to Specific Disorders of Spermatid Elongation via Multiple Metabolic Pathways inACS omega · 2024Article
- The Origin and Evolution of Sex Peptide and Sex Peptide Receptor Interactions.Molecular biology and evolution · 2024Article
- The Origin and Evolution of Sex Peptide and Sex Peptide Receptor Interactions.bioRxiv : the preprint server for biology · 2024Article
- A genetic toolkit underlying the queen phenotype in termites with totipotent workers.Scientific reports · 2024Article
- The seminal odorant binding protein Obp56g is required for mating plug formation and male fertility ineLife · 2023Article
- Evolutionary Quantitative Proteomics of Reproductive Protein Divergence in Drosophila.Molecular & cellular proteomics : MCP · 2023Article
- Single-cell RNA sequencing reveals cell landscape following antimony exposure during spermatogenesis in Drosophila testes.Cell death discovery · 2023Article
- Article
- Functional Divergence of the bag-of-marbles Gene in the Drosophila melanogaster Species Group.Molecular biology and evolution · 2022Article
- Characterization of reproductive proteins in the Mexican fruit fly points towards the evolution of novel functions.Proceedings. Biological sciences · 2022Article
- Single-nucleus transcriptomes reveal evolutionary and functional properties of cell types in the Drosophila accessory gland.Genetics · 2022Article
28 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors.
Funding
Abstract
As genomic sequences become easier to acquire, shotgun proteomics will play an increasingly important role in genome annotation. With proteomics, researchers can confirm and revise existing genome annotations and discover completely new genes. Proteomic-based de novo gene discovery should be especially useful for sets of genes with characteristics that make them difficult to predict with gene-finding algorithms. Here, we report the proteomic discovery of 19 previously unannotated genes encoding seminal fluid proteins (Sfps) that are transferred from males to females during mating in Drosophila. Using bioinformatics, we detected putative orthologs of these genes, as well as 19 others detected by the same method in a previous study, across several related species. Gene expression analysis revealed that nearly all predicted orthologs are transcribed and that most are expressed in a male-specific or male-biased manner. We suggest several reasons why these genes escaped computational prediction. Like annotated Sfps, many of these new proteins show a pattern of adaptive evolution, consistent with their potential role in influencing male sperm competitive ability. However, in contrast to annotated Sfps, these new genes are shorter, have a higher rate of nonsynonymous substitution, and have a markedly lower GC content in coding regions. Our data demonstrate the utility of applying proteomic gene discovery methods to a specific biological process and provide a more complete picture of the molecules that are critical to reproductive success in Drosophila.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.