ArticleApplied and environmental microbiology2009
New insights into {gamma}-aminobutyric acid catabolism: Evidence for {gamma}-hydroxybutyric acid and polyhydroxybutyrate synthesis in Saccharomyces cerevisiae.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 80 citations in OpenAlex.
- Stress-Driven Production of γ-Aminobutyric Acid Using Non-Conventional Yeast StrainsJournal of fungi (Basel, Switzerland) · 2024Article
- Proteomic changes orchestrate metabolic acclimation of a unicellular diazotrophic cyanobacterium during light-dark cycle and nitrogen fixation states.bioRxiv : the preprint server for biology · 2024Article
- Evaluation of Gamma-Aminobutyric Acid (GABA) as a Functional Feed Ingredient on Growth Performance, Immune Enhancement, and Disease Resistance in Olive Flounder (Antioxidants (Basel, Switzerland) · 2024Article
- TorC1 and nitrogen catabolite repression control of integrated GABA shunt and retrograde pathway gene expression.Yeast (Chichester, England) · 2023Article
- Enhance Production of γ-Aminobutyric Acid (GABA) and Improve the Function of Fermented Quinoa by Cold Stress.Foods (Basel, Switzerland) · 2022Article
- Transcriptome Analysis Unveils the Effects of Proline on Gene Expression in the YeastMicroorganisms · 2021Article
- Integration of Proteomics and Metabolomics Into the Design, Build, Test, Learn Cycle to Improve 3-Hydroxypropionic Acid Production inFrontiers in bioengineering and biotechnology · 2021Article
- Transcriptomic and Metabolomic Analysis Revealed Roles of Yck2 in Carbon Metabolism and Morphogenesis ofFrontiers in cellular and infection microbiology · 2021Article
- Detection of Fungi and Oomycetes by Volatiles Using E-Nose and SPME-GC/MS Platforms.Molecules (Basel, Switzerland) · 2020Article
- A Targeted Bioinformatics Assessment of Adrenocortical Carcinoma Reveals Prognostic Implications of GABA System Gene Expression.International journal of molecular sciences · 2020Article
- Regulation of Amino Acid Transport in Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 2019Review
- Comparison of pathway analysis and constraint-based methods for cell factory design.BMC bioinformatics · 2019Article
- The pleiotropic effects of the glutamate dehydrogenase (GDH) pathway in Saccharomyces cerevisiae.Microbial cell factories · 2018Review
- Promiscuous activities of heterologous enzymes lead to unintended metabolic rerouting inBiotechnology for biofuels · 2018Article
- A fungal transcription factor essential for starch degradation affects integration of carbon and nitrogen metabolism.PLoS genetics · 2017Article
- Phenotypic and chemotypic studies using Arabidopsis and yeast reveal that GHB converts to SSA and induce toxicity.Plant molecular biology · 2016Article
- Transcriptomic analysis of cobalt stress in the marine yeast Debaryomyces hansenii.FEMS yeast research · 2015Article
- An alternative, arginase-independent pathway for arginine metabolism in Kluyveromyces lactis involves guanidinobutyrase as a key enzyme.Molecular microbiology · 2014Article
- Defects in GABA metabolism affect selective autophagy pathways and are alleviated by mTOR inhibition.EMBO molecular medicine · 2014Article
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Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gamma-aminobutyrate (GABA) shunt, an alternative route for the conversion of alpha-ketoglutarate to succinate, involves the glutamate decarboxylase Gad1p, the GABA transaminase Uga1p and the succinate semialdehyde dehydrogenase Uga2p. This pathway has been extensively described in plants and animals, but its function in yeast remains unclear. We show that the flux through Gad1p is insignificant during fermentation in rich sugar-containing medium, excluding a role for this pathway in redox homeostasis under anaerobic conditions or sugar stress. However, we found that up to 4 g of exogenous GABA/liter was efficiently consumed by yeast. We studied the fate of this consumed GABA. Most was converted into succinate, with a reaction yield of 0.7 mol/mol. We also showed that a large proportion of GABA was stored within cells, indicating a possible role for this molecule in stress tolerance mechanisms or nitrogen storage. Furthermore, based on enzymatic and metabolic evidence, we identified an alternative route for GABA catabolism, involving the reduction of succinate-semialdehyde into gamma-hydroxybutyric acid and the polymerization of gamma-hydroxybutyric acid to form poly-(3-hydroxybutyric acid-co-4-hydroxybutyric acid). This study provides the first demonstration of a native route for the formation of this polymer in yeast. Our findings shed new light on the GABA pathway and open up new opportunities for industrial applications.
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