ArticleGenes & nutrition2009
Variation in protein levels obtained from human blood cells and biofluids for platelet, peripheral blood mononuclear cell, plasma, urine and saliva proteomics.
Article in Genes & nutrition, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- Modelling HIV-1 2-LTR dynamics following raltegravir intensification.Journal of the Royal Society, Interface · 2013Trial
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- Combined small and large magnetic nanoparticle extraction and concentration from biofluids for non-toxic detection of biomarkers.Sensors & diagnostics · 2022Article
- Clinical disease progression and biomarkers in Niemann-Pick disease type C: a prospective cohort study.Orphanet journal of rare diseases · 2020Observational
- Salivary lubricity (ex vivo) enhances upon moderate exercise: A pilot study.Archives of oral biology · 2020Article
- Characterization of low-dose ozone-induced murine acute lung injury.Physiological reports · 2020Article
- Comparison of inflammatory urine markers in patients with interstitial cystitis and overactive bladder.International urogynecology journal · 2018Article
- Disposable aptamer-sensor aided by magnetic nanoparticle enrichment for detection of salivary cortisol variations in obstructive sleep apnea patients.Scientific reports · 2017Article
- Investigation of Ion Transmission Effects on Intact Protein Quantification in a Triple Quadrupole Mass Spectrometer.Journal of the American Society for Mass Spectrometry · 2017Article
- Multiple Reaction Monitoring for Direct Quantitation of Intact Proteins Using a Triple Quadrupole Mass Spectrometer.Journal of the American Society for Mass Spectrometry · 2016Article
- Leukopak PBMC sample processing for preparing quality control material to support proficiency testing programs.Journal of immunological methods · 2014Article
- Spatial modeling of HIV cryptic viremia and 2-LTR formation during raltegravir intensification.Journal of theoretical biology · 2014Article
- Assessment of p38α in peripheral blood mononuclear cells (PBMC): a potential blood protein marker of head and neck squamous cell carcinoma.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2013Article
- Perspective: a systems approach to diabetes research.Frontiers in genetics · 2013Review
- Influence of topography and hydrophilicity on initial oral biofilm formation on microstructured titanium surfaces in vitro.Clinical oral implants research · 2012Article
- 2D-electrophoresis and multiplex immunoassay proteomic analysis of different body fluids and cellular components reveal known and novel markers for extended fasting.BMC medical genomics · 2011Article
- Estimating trace deposition time with circadian biomarkers: a prospective and versatile tool for crime scene reconstruction.International journal of legal medicine · 2010Article
- Urine collection and processing for protein biomarker discovery and quantification.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2010Review
- Urine biomarker could be a useful tool for differential diagnosis of a lower urinary tract dysfunction.Tzu chi medical journalReview
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Blood cells and biofluid proteomics are emerging as a valuable tool to assess effects of interventions on health and disease. This study is aimed to assess the amount and variability of proteins from platelets, peripheral blood mononuclear cells (PBMC), plasma, urine and saliva from ten healthy volunteers for proteomics analysis, and whether protein yield is affected by prolonged fasting. Volunteers provided blood, saliva and morning urine samples once a week for 4 weeks after an overnight fast. Volunteers were fasted for a further 24 h after the fourth sampling before providing their final samples. Each 10 mL whole blood provided 400-1,500 mug protein from platelets, and 100-600 mug from PBMC. 30 muL plasma depleted of albumin and IgG provided 350-650 mug protein. A sample of morning urine provided 0.9-8.6 mg protein/dL, and a sample of saliva provided 70-950 mug protein/mL. None of these yields were influenced by the degree of fasting (overnight or 36 h). In conclusion, in contrast to the yields from plasma, platelets and PBMC, the protein yields of urine and saliva samples were highly variable within and between subjects. Certain disease conditions may cause higher or lower PBMC counts and thus protein yields, or increased urinary protein levels.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.