ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2009
Role of lysophosphatidylcholine in brush-border intestinal alkaline phosphatase release and restoration.
Article in American journal of physiology. Gastrointestinal and liver physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 52 citations in OpenAlex.
- Intestinal metabolomics analysis reveals lysophosphatidylcholine is the key regulatory metabolite in mouse models of necrotizing enterocolitis.Scientific reports · 2025Article
- Novel Role of theInternational journal of molecular sciences · 2024Article
- Intestinal Alkaline Phosphatase: A Review of This Enzyme Role in the Intestinal Barrier Function.Microorganisms · 2022Review
- Primary exploration of host-microorganism interaction and enteritis treatment with an embedded membrane microfluidic chip of the human intestinal-vascular microsystem.Frontiers in bioengineering and biotechnology · 2022Article
- The Effect of Bacterial Infections, Probiotics and Zonulin on Intestinal Barrier Integrity.International journal of molecular sciences · 2021Review
- Uptake of Vitamins DNutrients · 2021Article
- Article
- Development of chimeric peptides to facilitate the neutralisation of lipopolysaccharides during bactericidal targeting of multidrug-resistant Escherichia coli.Communications biology · 2020Article
- Duodenal chemosensing.Current opinion in gastroenterology · 2018Review
- Leaky Gut As a Danger Signal for Autoimmune Diseases.Frontiers in immunology · 2017Review
- Intestinal alkaline phosphatase: a summary of its role in clinical disease.The Journal of surgical research · 2016Review
- Ezetimibe Promotes Brush Border Membrane-to-Lumen Cholesterol Efflux in the Small Intestine.PloS one · 2016Article
- Glyceroglycolipids Affect Uptake of Carotenoids Solubilized in Mixed Micelles by Human Intestinal Caco-2 Cells.Lipids · 2015Article
- Cellular function and molecular structure of ecto-nucleotidases.Purinergic signalling · 2012Review
- Crucial role of alkaline sphingomyelinase in sphingomyelin digestion: a study on enzyme knockout mice.Journal of lipid research · 2011Article
- Intestinal alkaline phosphatase has beneficial effects in mouse models of chronic colitis.Inflammatory bowel diseases · 2011Article
- If Hoofbeats are not From Horses, It Could be Zebras!! Isolated Hyper-alkaline Phosphatasemia.Journal of clinical and experimental hepatologyArticle
Corrections and comments
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Authors and funding
13 authors at 1 institution in 1 country.
Funding
Abstract
Intestinal alkaline phosphatase (IAP) is a brush-border membrane ectoenzyme (BBM-IAP) that is released into the lumen (L-IAP) after a high-fat diet. We examined the effects of oil feeding and the addition of mixed-lipid micelles on the formation of L-IAP in oil-fed rat intestine, Caco-2 cell monolayers, and mouse intestinal loops. We localized IAP in the duodenum of rats fed corn oil using fluorescence microscopy with enzyme-labeled fluorescence-97 as substrate. Four hours after oil feeding, L-IAP increased approximately 10-fold accompanied by the loss of BBM-IAP, consistent with BBM-IAP release. Rat IAP isozyme mRNAs progressively increased 4-6 h after oil feeding, followed by the increase of IAP activity in the subapical location at 6 h, consistent with the restoration of IAP protein. Postprandial lipid-micelle components, sodium taurocholate with or without oleic acid, mono-oleylglycerol, cholesterol, or lysophosphatidylcholine (lysoPC) were applied singly or as mixed-lipid micelles to the apical surface of polarized Caco-2 cell monolayers. LysoPC increased L-IAP >10-fold over basal release. LysoPC released IAP into the apical medium more than other intestinal brush-border enzymes, 5'-nucleotidase, sucrase, aminopeptidase N, and lactase, without comparable lactate dehydrogenase release or cell injury. LysoPC increased human IAP mRNA levels by 1.5-fold in Caco-2 cells. Luminally applied lysoPC also increased release of IAP preferentially in mouse intestinal loops. These data show that lysoPC accelerates the formation of L-IAP from BBM-IAP, followed by enhanced IAP synthesis, suggesting the role that lysoPC might play in the turnover of brush-border proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.