ArticleInvestigative ophthalmology & visual science2009
Mucin-type O-glycans in tears of normal subjects and patients with non-Sjögren's dry eye.
Article in Investigative ophthalmology & visual science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 52 citations in OpenAlex.
- In-depth analysis of the tear fluid glycoproteome reveals diverse lacritin glycosylation and spliceoforms.The Journal of biological chemistry · 2025Article
- In-depth analysis of the tear fluid glycoproteome reveals diverse lacritin glycosylation and spliceoforms.bioRxiv : the preprint server for biology · 2025Article
- Ocular surface glycocalyx in health and disease.Frontiers in cell and developmental biology · 2025Review
- Altered O-Glycans in stimulated whole saliva from patients with primary Sjögren's syndrome and non-pSS sicca.Scientific reports · 2024Article
- Ectoine Enhances Mucin Production Via Restoring IL-13/IFN-γ Balance in a Murine Dry Eye Model.Investigative ophthalmology & visual science · 2024Article
- Characterization of Cell Surface Glycan Profiles in Human and Mouse Corneas Using Lectin Microarrays.Cells · 2023Article
- Fungal dysbiosis and decreased tear mucin at the conjunctiva in patients with conjunctival mucosa-associated lymphoid tissue lymphoma.BMJ open ophthalmology · 2023Article
- In-depth quantitative proteomics analysis revealed C1GALT1 depletion in ECC-1 cells mimics an aggressive endometrial cancer phenotype observed in cancer patients with low C1GALT1 expression.Cellular oncology (Dordrecht, Netherlands) · 2023Article
- The protective role of conjunctival goblet cell mucin sialylation.Nature communications · 2023Article
- Human ocular mucins: The endowed guardians of sight.Advanced drug delivery reviews · 2022Review
- Development of a HPLC-MS/MS method for assessment of thiol redox status in human tear fluids.Analytical biochemistry · 2021Article
- Inhibition of Aberrant α(1,2)-Fucosylation at Ocular Surface Ameliorates Dry Eye Disease.International journal of molecular sciences · 2021Article
- Membrane-associated mucins of the human ocular surface in health and disease.The ocular surface · 2021Review
- Glycosylation pathways of human corneal and conjunctival epithelial cell mucins.Carbohydrate research · 2018Article
- Glycosylation pathways at the ocular surface.Biochemical Society transactions · 2018Review
- Proteomic analysis of human lacrimal and tear fluid in dry eye disease.Scientific reports · 2017Article
- Eukaryotic protein glycosylation: a primer for histochemists and cell biologists.Histochemistry and cell biology · 2017Review
- Cryptic de novo deletion at 2q23.3-q24.1 in a patient with intellectual disability.Journal of genetics · 2016Article
- Cocaine snorting may induce ocular surface damage through corneal sensitivity impairment.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2015Article
- Genome sequencing of the perciform fish Larimichthys crocea provides insights into molecular and genetic mechanisms of stress adaptation.PLoS genetics · 2015Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
purposeO-linked carbohydrates (O-glycans) contribute to the hydrophilic character of mucins in mucosal tissues. This study was conducted to identify the repertoire of O-glycans in the tear film and the glycosyltransferases associated with their biosynthesis, in normal subjects and patients with non-Sjögren's dry eye.
methodsHuman tear fluid was collected from the inferior conjunctival fornix. O-glycans were released by hydrazinolysis, labeled with 2-aminobenzamide, and analyzed by fluorometric, high-performance liquid chromatography (HPLC) coupled with exoglycosidase digestions. O-glycan structures identified in tears were related to potential biosynthetic pathways in human conjunctival epithelium by using a glycogene microarray database. Lectin-binding analyses were performed with agglutinins from Arachis hypogaea, Maackia amurensis, and Sambucus nigra.
resultsThe O-glycan profile of human tears consisted primarily of core 1 (Gal beta 1-3GalNAc alpha 1-Ser/Thr)-based structures. Mono-sialyl O-glycans represented approximately 66% of the glycan pool, with alpha2-6-sialyl core 1 being the predominant O-glycan structure in human tears (48%). Four families of glycosyltransferases potentially related to the biosynthesis of these structures were identified in human conjunctiva. These included 13 polypeptide-GalNAc-transferases (GALNT), the core 1 beta-3-galactosyltransferase (T-synthase), three alpha2-6-sialyltransferases (ST6GalNAc), and two alpha2-3-sialyltransferases (ST3Gal). No significant differences in total amount of O-glycans were detected between tears of normal subjects and patients with dry eye, by HPLC and lectin blot. Likewise, no differences in glycosyltransferase expression were found by glycogene microarray.
conclusionsThis study identified the most common mucin-type O-glycans in human tears and their expected biosynthetic pathways in ocular surface epithelia. Patients with non-Sjögren's dry eye showed no alterations in composition and amount of O-glycans in the tear fluid.
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