ArticleBiochimica et biophysica acta2009
A dual mechanism of cytoprotection afforded by M-LDH in embryonic heart H9C2 cells.
Article in Biochimica et biophysica acta, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Increase in cardioprotective SUR2A does not alter heart rate and heart rate regulation by physical activity and diurnal rhythm.Journal of basic and clinical physiology and pharmacology · 2022Article
- SUR2A as a base for cardioprotective therapeutic strategies.Molecular biology reports · 2022Review
- Isosteviol prevents the development of isoprenaline‑induced myocardial hypertrophy.International journal of molecular medicine · 2019Article
- Pyrazinamide may possess cardioprotective properties.The Journal of antibiotics · 2019Article
- Insulin down-regulates cardioprotective SUR2A in the heart-derived H9c2 cells: A possible explanation for some adverse effects of insulin therapy.Biochemistry and biophysics reports · 2018Article
- The Citrus Flavanone Naringenin Protects Myocardial Cells against Age-Associated Damage.Oxidative medicine and cellular longevity · 2017Article
- Phenylephrine preconditioning in embryonic heart H9c2 cells is mediated by up-regulation of SUR2B/Kir6.2: A first evidence for functional role of SUR2B in sarcolemmal KATP channels and cardioprotection.The international journal of biochemistry & cell biology · 2016Article
- Mild hypoxia in vivo regulates cardioprotective SUR2A: A role for Akt and LDH.Biochimica et biophysica acta · 2015Article
- Upregulation of cardioprotective SUR2A by sub-hypoxic drop in oxygen.Biochimica et biophysica acta · 2014Article
- Testosterone protects female embryonic heart H9c2 cells against severe metabolic stress by activating estrogen receptors and up-regulating IES SUR2B.The international journal of biochemistry & cell biology · 2013Article
- Measuring mitochondrial function in intact cardiac myocytes.Journal of molecular and cellular cardiology · 2012Review
- Human oocytes express ATP-sensitive K(+) channels.Human reproduction (Oxford, England) · 2010Article
- Nicotinamide-rich diet protects the heart against ischaemia-reperfusion in mice: a crucial role for cardiac SUR2A.Pharmacological research · 2010Article
- Infection with AV-SUR2A protects H9C2 cells against metabolic stress: a mechanism of SUR2A-mediated cytoprotection independent from the K(ATP) channel activity.Biochimica et biophysica acta · 2010Article
- M-LDH physically associated with sarcolemmal K ATP channels mediates cytoprotection in heart embryonic H9C2 cells.The international journal of biochemistry & cell biology · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Muscle form of lactate dehydrogenase (M-LDH), a minor LDH form in cardiomyocytes, physically interacts with ATP-sensitive K+ (K ATP) channel-forming subunits. Here, we have shown that expression of 193gly-M-LDH, an inactive mutant of M-LDH, inhibit regulation of the K ATP channels activity by LDH substrates in embryonic rat heart H9C2 cells. In cells expressing 193gly-M-LDH chemical hypoxia has failed to activate K ATP channels. The similar results were obtained in H9C2 cells expressing Kir6.2AFA, a mutant form of Kir6.2 with largely decreased K+ conductance. Kir6.2AFA has slightly, but significantly, reduced cellular survival under chemical hypoxia while the deleterious effect of 193gly-M-LDH was significantly more pronounced. The levels of total and subsarcolemmal ATP in H9C2 cells were not affected by Kir6.2AFA, but the expression of 193gly-M-LDH led to lower levels of subsarcolemmal ATP during chemical hypoxia. We conclude that M-LDH regulates both the channel activity and the levels of subsarcolemmal ATP and that both mechanism contribute to the M-LDH-mediated cytoprotection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.