ArticleJournal of virological methods2009
Broadly targeted multiprobe QPCR for detection of coronaviruses: Coronavirus is common among mallard ducks (Anas platyrhynchos).
Article in Journal of virological methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 53 citations in OpenAlex.
- Avian influenza and coronaviruses in live animal and wet markets in Laos: prevalence and public health considerations.Frontiers in cellular and infection microbiology · 2026Article
- Novel multiplex family-wide PCR and Nanopore sequencing of amplicons (FP-NSA) approach for surveillance of influenza- and coronaviruses in humans and animals.Genome medicine · 2025Article
- Gulls as a host for both gamma and deltacoronaviruses.Scientific reports · 2023Article
- SARS-CoV-2 Surveillance between 2020 and 2021 of All Mammalian Species in Two Flemish Zoos (Antwerp Zoo and Planckendael Zoo).Veterinary sciences · 2023Article
- Synchronicity of viral shedding in molossid bat maternity colonies.Epidemiology and infection · 2023Article
- The Role of Molossidae and Vespertilionidae in Shaping the Diversity of Alphacoronaviruses in the Americas.Microbiology spectrum · 2022Article
- Molecular Survey on A, B, C and New Avian Metapneumovirus (aMPV) Subtypes in Wild Birds of Northern-Central Italy.Veterinary sciences · 2022Article
- Monitoring Urban Zoonotic Virus Activity: Are City Rats a Promising Surveillance Tool for Emerging Viruses?Viruses · 2022Article
- Seasonality of coronavirus shedding in tropical bats.Royal Society open science · 2022Article
- Transmission of SARS-CoV-2 from Human to Domestic Ferret.Emerging infectious diseases · 2021Article
- Detection of Laryngotracheitis Virus in Poultry Flocks with Respiratory Disorders in Slovenia.Viruses · 2021Article
- Coronaviruses in wild birds - A potential and suitable vector for global distribution.Veterinary medicine and science · 2021Review
- Wild birds as reservoirs for diverse and abundant gamma- and deltacoronaviruses.FEMS microbiology reviews · 2020Review
- Bat coronavirus phylogeography in the Western Indian Ocean.Scientific reports · 2020Article
- Discovery and Characterization of Novel RNA Viruses in Aquatic North American Wild Birds.Viruses · 2019Article
- Coronaviruses in Avian Species - Review with Focus on Epidemiology and Diagnosis in Wild Birds.Journal of veterinary research · 2018Article
- Detection and characterisation of coronaviruses in migratory and non-migratory Australian wild birds.Scientific reports · 2018Article
- Article
- Urbanization and the dynamics of RNA viruses in Mallards (Anas platyrhynchos).Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2017Article
- Prevalence and genetic diversity of coronaviruses in wild birds, Finland.Infection ecology & epidemiology · 2017Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coronaviruses (CoVs) can cause trivial or fatal disease in humans and in animals. Detection methods for a wide range of CoVs are needed, to understand viral evolution, host range, transmission and maintenance in reservoirs. A new concept, "Multiprobe QPCR", which uses a balanced mixture of competing discrete non- or moderately degenerated nuclease degradable (TaqMan) probes was employed. It provides a broadly targeted and rational single tube real-time reverse transcription PCR ("NQPCR") for the generic detection and discovery of CoV. Degenerate primers, previously published, and the new probes, were from a conserved stretch of open reading frame 1b, encoding the replicase. This multiprobe design reduced the degree of probe degeneration, which otherwise decreases the sensitivity, and allowed a preliminary classification of the amplified sequence directly from the QPCR trace. The split probe strategy allowed detection of down to 10 viral nucleic acid equivalents of CoV from all known CoV groups. Evaluation was with reference CoV strains, synthetic targets, human respiratory samples and avian fecal samples. Infectious-Bronchitis-Virus (IBV)-related variants were found in 7 of 35 sample pools, from 100 wild mallards (Anas platyrhynchos). Ducks may spread and harbour CoVs. NQPCR can detect a wide range of CoVs, as illustrated for humans and ducks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.