ArticleJournal of virological methods2009
Accurate single-day titration of adenovirus vectors based on equivalence of protein VII nuclear dots and infectious particles.
Article in Journal of virological methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Post-translational modifications on protein VII are important during the early stages of adenovirus infection.Journal of virology · 2025Article
- Virus Infection Variability by Single-Cell Profiling.Viruses · 2021Review
- Adenovirus Terminal Protein Contains a Bipartite Nuclear Localisation Signal Essential for Its Import into the Nucleus.International journal of molecular sciences · 2021Article
- Adenovirus Core Proteins: Structure and Function.Viruses · 2021Review
- Article
- The adenovirus major core protein VII is dispensable for virion assembly but is essential for lytic infection.PLoS pathogens · 2017Article
- Review
- The influence of stabilizers and rates of freezing on preserving of structurally different animal viruses during lyophilization and subsequent storage.Journal of applied microbiology · 2014Article
- Review
- A direct and versatile assay measuring membrane penetration of adenovirus in single cells.Journal of virology · 2013Article
- The role of chromatin in adenoviral vector function.Viruses · 2013Review
- SPOC1-mediated antiviral host cell response is antagonized early in human adenovirus type 5 infection.PLoS pathogens · 2013Article
- Chromatin structure of adenovirus DNA throughout infection.Nucleic acids research · 2012Review
- Adenovirus-triggered innate signalling pathways.European journal of microbiology & immunology · 2011Review
- Stepwise loss of fluorescent core protein V from human adenovirus during entry into cells.Journal of virology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Protein VII is an abundant component of adenovirus particles and is tightly associated with the viral DNA. It enters the nucleus along with the infecting viral genome and remains bound throughout early phase. Protein VII can be visualized by immunofluorescent staining as discrete dots in the infected cell nucleus. Comparison between protein VII staining and expression of the 72kDa DNA-binding protein revealed a one-to-one correspondence between protein VII dots and infectious viral genomes. A similar relationship was observed for a helper-dependent adenovirus vector expressing green fluorescent protein. This relationship allowed accurate titration of adenovirus preparations, including wild-type and helper-dependent vectors, using a 1-day immunofluorescence method. The method can be applied to any adenovirus vector and gives results equivalent to the standard plaque assay.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.