ArticleJournal of periodontology2009
Expression profile of human gingival fibroblasts induced by interleukin-1beta reveals central role of nuclear factor-kappa B in stabilizing human gingival fibroblasts during inflammation.
Article in Journal of periodontology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 43 citations in OpenAlex.
- Investigating the causal impact of gut microbiota on periodontitis based on Mendelian randomization, bulk transcriptomics, and single-cell transcriptomics.Frontiers in cell and developmental biology · 2026Article
- Recombinant Human Keratinocyte Growth Factor Ameliorates Cancer Treatment-Induced Oral Mucositis on a Chip.Advanced healthcare materials · 2024Article
- Oral mucositis on a chip: modeling induction by chemo- and radiation treatments and recovery.Biofabrication · 2022Article
- The Influence of Periodontal Diseases and the Stimulation of Saliva Secretion on the Course of the Acute Phase of Ischemic Stroke.Journal of clinical medicine · 2022Article
- Microbiota in Periodontitis: Advances in the Omic Era.Advances in experimental medicine and biology · 2022Review
- Multi-Omics Analysis of Anti-Inflammatory Action of Alkaline Extract of the Leaves ofJournal of clinical medicine · 2021Article
- An Oral-mucosa-on-a-chip sensitively evaluates cell responses to dental monomers.Biomedical microdevices · 2021Article
- Hyperoside ameliorates periodontitis in rats by promoting osteogenic differentiation of BMSCs via activation of the NF-κB pathway.FEBS open bio · 2020Article
- Quantitative scoring of epithelial and mesenchymal qualities of cancer cells using machine learning and quantitative phase imaging.Journal of biomedical optics · 2020Article
- Machine Learning with Optical Phase Signatures for Phenotypic Profiling of Cell Lines.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2019Article
- Oral mucosa-on-a-chip to assess layer-specific responses to bacteria and dental materials.Biomicrofluidics · 2018Article
- DPIE [2-(1,2-diphenyl-1H-indol-3-yl)ethanamine] Augments Pro-Inflammatory Cytokine Production in IL-1β-Stimulated Primary Human Oral Cells.International journal of molecular sciences · 2018Article
- IL-1β-induced modulation of gene expression profile in human dermal fibroblasts: the effects of Thai herbal Sahatsatara formula, piperine and gallic acid possessing antioxidant properties.BMC complementary and alternative medicine · 2017Article
- Environmental stimuli shape biofilm formation and the virulence of periodontal pathogens.International journal of molecular sciences · 2013Review
- Attenuation of inflammatory mediator production by the NF-κB member RelB is mediated by microRNA-146a in lung fibroblasts.American journal of physiology. Lung cellular and molecular physiology · 2013Article
- Atypical IκB proteins - nuclear modulators of NF-κB signaling.Cell communication and signaling : CCS · 2013Article
- Potential immune modularly role of glycine in oral gingival inflammation.Clinical & developmental immunology · 2013Article
- Article
- Proteomics as it pertains to oral pathologies and dental research.Bioinformation · 2011Article
- Association of cytokine gene alleles with the inflammation of human periodontal tissue.Acta naturae · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundInterleukin (IL)-1beta is a key cytokine in the pathogenesis of periodontitis, and it induces inflammatory mediators in periodontal diseases. We developed immortalized human gingival fibroblasts (HGFs), investigated the effects of IL-1beta on the gene expression using expression arrays containing approximately 40,000 genes, and tested the role of nuclear factor-kappa B (NF-kappaB) in maintaining an activated HGF population.
methodsTotal RNA was isolated from IL-1beta-induced and mock-induced control cells. Gene expression analyses were performed using expression arrays and confirmed by quantitative real-time polymerase chain reaction. Western blot analysis to show inhibitor of kappa B-alpha (IkappaBalpha) phosphorylation and immunostaining of cells for NF-kappaB nuclear translocation were performed. Apoptosis was confirmed by assay of poly ADP-ribose polymerase (PARP) cleavage.
resultsA total of 382 probe sets corresponding to 254 genes were differentially expressed in IL-1beta-induced cells (P <0.001). A total of 215 genes were upregulated, and 39 genes were downregulated. Most notable NF-kappaB pathway members (NFkappaB1, NFkappaB2, IkappaBalpha, IkappaBepsilon, IkappaBzeta, REL, RELB, and TA-NFKBH) were upregulated. IkappaBalpha was phosphorylated, and NF-kappaB accumulated in the nucleus. An IL-1beta-induced set of 27 genes was downregulated by an NF-kappaB inhibitor, leading to a decreased number of viable cells and suggesting an antiapoptotic role for NF-kappaB.
conclusionsIL-1beta leads to a large number of significant expression changes consistent with a pathologic role in periodontitis, including enhancement of inflammatory cytokines, chemokines, transcription factors, matrix metalloproteinases, adhesion molecules, and especially NF-kappaB-dependent antiapoptotic genes. NF-kappaB activation blocks apoptosis, thereby stabilizing the HGF population in inflammation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.