ArticleEuropean biophysics journal : EBJ2009
Determination of axonal transport velocities via image cross- and autocorrelation.
Article in European biophysics journal : EBJ, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- KymographClear and KymographDirect: two tools for the automated quantitative analysis of molecular and cellular dynamics using kymographs.Molecular biology of the cell · 2016Article
- Automated Multi-Peak Tracking Kymography (AMTraK): A Tool to Quantify Sub-Cellular Dynamics with Sub-Pixel Accuracy.PloS one · 2016Article
- Object tracking with particle filtering in fluorescence microscopy images: application to the motion of neurofilaments in axons.IEEE transactions on medical imaging · 2012Article
- A fast and robust method for automated analysis of axonal transport.European biophysics journal : EBJ · 2011Article
- Automated image analysis for tracking cargo transport in axons.Microscopy research and technique · 2011Article
Corrections and comments
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Authors and funding
7 authors.
Funding
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Abstract
On their way to the synapse and back, neuronal proteins are carried in cargo vesicles along axons and dendrites. Here, we demonstrate that the key parameters of axonal transport, i.e., particle velocities and pausing times can be read out from CCD-camera images automatically. In the present study, this is achieved via cross- and autocorrelation of kymograph columns. The applicability of the method was measured on simulated kymographs and data from axonal transport timeseries of mRFP-labeled synaptophysin. In comparing outcomes of velocity determinations via a performance parameter that is analogous to the signal-to-noise ratio (SNR) definition, we find that outcomes are dependent on sampling, particle numbers and signal to noise of the kymograph. Autocorrelation of individual columns allows exact determination of pausing time populations. In contrast to manual tracking, correlation does not require experience, a priori assumptions or disentangling of individual particle trajectories and can operate at low SNR.
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