ArticleHFSP journal2008
Quantitative analysis of fluorescence lifetime imaging made easy.
Article in HFSP journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed.
- Tunable Cytosolic Chloride Indicators for Real-Time Chloride Imaging in Live Cells.bioRxiv : the preprint server for biology · 2024Article
- Article
- Processing of fluorescence lifetime image using modified phasor approach: homo-FRET from the acceptor.Journal of fluorescence · 2013Article
- Efficient parallel Levenberg-Marquardt model fitting towards real-time automated parametric imaging microscopy.PloS one · 2013Article
- G-quadruplex structure and stability illuminated by 2-aminopurine phasor plots.Nucleic acids research · 2012Article
- Advanced fluorescence microscopy techniques--FRAP, FLIP, FLAP, FRET and FLIM.Molecules (Basel, Switzerland) · 2012Review
- Global analysis of dynamic fluorescence anisotropy by a polarized phasor approach.Journal of fluorescence · 2011Article
- Fluorescence lifetime measurements and biological imaging.Chemical reviews · 2010Review
- Endothelial adhesion receptors are recruited to adherent leukocytes by inclusion in preformed tetraspanin nanoplatforms.The Journal of cell biology · 2008Article
- FRET imaging of hemoglobin concentration in Plasmodium falciparum-infected red cells.PloS one · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence lifetime imaging is a valuable and versatile tool for the investigation of the molecular environment of fluorophores in living cells. It is ideally suited-and is therefore increasingly used-for the quantification of the occurrence of Förster Resonance Energy Transfer, a powerful microscopy method for the detection of subnanometer conformational changes, protein-protein interactions, and protein biochemical status. However, careful quantitative analysis is required for the correct and meaningful interpretation of fluorescence lifetime data. This can be a daunting task to the nonexpert user, and is the source for many avoidable errors and unsound interpretations. Digman and colleagues (Digman et al., 2007, Biophys. J. 94, L14-6) present an analysis technique that avoids data fitting in favor of a simple graphical polar data representation. In this "phasor" space, the physics of lifetime imaging becomes more intuitive and accessible also to the inexperienced user. The cumulated information from image pixels, even over different cells, describes patterns and trajectories that can be visually interpreted in physically meaningful ways. Its usefulness is demonstrated in the study of the dimerization of the uPAR receptor (Caiolfa et al., 2007, J. Cell Biol. 179, 1067-1082).
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.