ArticleNature2009
Precise genome modification in the crop species Zea mays using zinc-finger nucleases.
Article in Nature, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 288 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
288 citing papers in PubMed, 946 citations in OpenAlex.
- Plant viral suppressors HcPro and P19 inhibit CRISPR-Cas13-mediated transcript interference.Stress biology · 2026Article
- AI-assisted crop improvement: new design tools within established regulatory frameworks.Frontiers in plant science · 2026Article
- Genome editing beyond transgenes: DNA-free technologies in crop improvement.Frontiers in plant science · 2026Review
- Impact of genetically modified herbicide-resistant maize on rhizosphere bacterial communities.GM crops & food · 2025Article
- A single donor cassette enables site-specific knock-in at either the αAmy3 or αAmy8 locus in rice cells via CRISPR/Cas9.Applied microbiology and biotechnology · 2025Article
- Integrating genome editing with omics, artificial intelligence, and advanced farming technologies to increase crop productivity.Plant communications · 2025Review
- Emerging trends in transgene-free crop development: insights into genome editing and its regulatory overview.Plant molecular biology · 2025Review
- Directed mutagenesis in fruit crops.3 Biotech · 2025Review
- Beyond a few bases: methods for large DNA insertion and gene targeting in plants.The Plant journal : for cell and molecular biology · 2025Review
- Recent advances in site-specific transgene insertion into the maize genome using recombinases and genome editing endonucleases.Frontiers in plant science · 2025Review
- Development of efficient targeted insertion mediated by CRISPR-Cas12a and homology-directed repair in maize.Frontiers in genome editing · 2025Article
- Genome editing for grass improvement and future agriculture.Horticulture research · 2025Article
- Advancements in Plant Gene Editing Technology: From Construct Design to Enhanced Transformation Efficiency.Biotechnology journal · 2024Review
- Biotechnological approaches to reduce the phytic acid content in millets to improve nutritional quality.Planta · 2024Review
- CRISPR/Cas9-mediated genome editing technique to control fall armyworm (Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
- Regulation of gene-edited plants in Europe: from the valley of tears into the shining sun?aBIOTECH · 2024Review
- Recent Advances in Tomato Gene Editing.International journal of molecular sciences · 2024Review
- Precise integration of large DNA sequences in plant genomes using PrimeRoot editors.Nature biotechnology · 2024Article
- Breeding for improved digestibility and processing of lignocellulosic biomass inFrontiers in plant science · 2024Review
- An Overview of Targeted Genome Editing Strategies for Reducing the Biosynthesis of Phytic Acid: an Anti-nutrient in Crop Plants.Molecular biotechnology · 2024Review
228 more citing papers are in PubMed but not listed here.
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Authors and funding
25 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Agricultural biotechnology is limited by the inefficiencies of conventional random mutagenesis and transgenesis. Because targeted genome modification in plants has been intractable, plant trait engineering remains a laborious, time-consuming and unpredictable undertaking. Here we report a broadly applicable, versatile solution to this problem: the use of designed zinc-finger nucleases (ZFNs) that induce a double-stranded break at their target locus. We describe the use of ZFNs to modify endogenous loci in plants of the crop species Zea mays. We show that simultaneous expression of ZFNs and delivery of a simple heterologous donor molecule leads to precise targeted addition of an herbicide-tolerance gene at the intended locus in a significant number of isolated events. ZFN-modified maize plants faithfully transmit these genetic changes to the next generation. Insertional disruption of one target locus, IPK1, results in both herbicide tolerance and the expected alteration of the inositol phosphate profile in developing seeds. ZFNs can be used in any plant species amenable to DNA delivery; our results therefore establish a new strategy for plant genetic manipulation in basic science and agricultural applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.