ArticleJournal of clinical microbiology2009
Rapid molecular characterization of Clostridium difficile and assessment of populations of C. difficile in stool specimens.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 55 citations in OpenAlex.
- A novel RNA nucleic acid amplification test more accurately distinguishes activemedRxiv : the preprint server for health sciences · 2026Article
- Cross-species engraftment biases and metabolic divergence in gnotobiotic mice humanized with ulcerative colitis microbiota.Gut microbes · 2025Article
- Antibiotic resistance patterns, toxin profiles, and toxin gene expression levels among Clostridioides difficile PCR ribotypes isolated from patients scheduled for colonoscopy.Molecular biology reports · 2025Article
- The analysis of the epidemic status and risk factors associated with different genotypes Clostridioides difficile mixed infection.BMC microbiology · 2025Article
- Analysis of Clostridioides difficile Infection in Children with Diarrhea in Two Hospitals in Southern Brazil.Current microbiology · 2023Article
- Virulence and genomic diversity among clinical isolates of ST1 (BI/NAP1/027) Clostridioides difficile.Cell reports · 2023Article
- Establishment of a Novel Detection Platform for Clostridioides difficile Toxin Genes Based on Orthogonal CRISPR.Microbiology spectrum · 2023Article
- Laboratory diagnosis of Clostridioides difficile infection in symptomatic patients: what can we do better?Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Virulence and genomic diversity among clinical isolates of ST1 (BI/NAP1/027)bioRxiv : the preprint server for biology · 2023Article
- Binary Toxin Expression byOpen forum infectious diseases · 2022Article
- Comparative biofilm-forming ability betweenFrontiers in cellular and infection microbiology · 2022Article
- Comparison of clinical severity, genotype and toxin gene expression of binary toxin-producingAccess microbiology · 2022Article
- Article
- Presence of multiple Clostridium difficile strains at primary infection is associated with development of recurrent disease.Anaerobe · 2018Article
- Toxigenic Clostridium difficile colonization among hospitalised adults; risk factors and impact on survival.The Journal of infection · 2017Observational
- Article
- Prevalence of binary toxin positive Clostridium difficile in diarrhoeal humans in the absence of epidemic ribotype 027.PloS one · 2017Article
- Premarket evaluations of the IMDx C. difficile for Abbott m2000 Assay and the BD Max Cdiff Assay.Journal of clinical microbiology · 2014Article
- Survey ofInternational journal of clinical medicine · 2014Article
- Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea.Scientifica · 2014Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Our laboratory has developed testing methods that use real-time PCR and pyrosequencing analysis to enable the rapid identification of potential hypervirulent Clostridium difficile strains. We describe a real-time PCR assay that detects four C. difficile genes encoding toxins A (tcdA) and B (tcdB) and the binary toxin genes (cdtA and cdtB), as well as a pyrosequencing assay that detects common deletions in the tcdC gene in less than 4 h. A subset of historical and recent C. difficile isolates (n = 31) was also analyzed by pulsed-field gel electrophoresis to determine the circulating North American pulsed-field (NAP) types that have been isolated in New York State. Thirteen different NAP types were found among the 31 isolates tested, 13 of which were NAP type 1 strains. To further assess the best approach to utilizing our conventional and molecular methods, we studied the populations of C. difficile in patient stool specimens (n = 23). Our results indicated that 13% of individual stool specimens had heterogeneous populations of C. difficile when we compared the molecular characterization results for multiple bacterial isolates (n = 10). Direct molecular analysis of stool specimens gave results that correlated well with the results obtained with cultured stool specimens; the direct molecular analysis was rapid, informative, and less costly than the testing of multiple patient stool isolates.
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