Evidence map›Paper›PMID 19403766›Full record

ArticleJournal of clinical microbiology2009

Aspergillus section Fumigati typing by PCR-restriction fragment polymorphism.

Janet F Staab, S Arunmozhi Balajee, Kieren A Marr

Abstract readValidation Study
In one paragraph

Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed.

  1. Identification ofJournal of clinical microbiology · 2026
    Article
  2. First case of fungal peritonitis caused byMedical mycology case reports · 2024
    Article
  3. Article
  4. Article
  5. Azole-ResistantMicroorganisms · 2020
    Article
  6. Aspergillosis in free-ranging aquatic birds.Medical mycology case reports · 2020
    Article
  7. Article
  8. Article
  9. Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. Is azole resistance in Aspergillus fumigatus a problem in Spain?Antimicrobial agents and chemotherapy · 2013
    Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Janet F StaabJohns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
S Arunmozhi Balajee
Kieren A Marr

Funding

Genetic Diversity of A. fumigatusR21AI067971 · NIAID · OREGON HEALTH & SCIENCE UNIVERSITY · PI MARR, KIEREN A. · 2006 to 2007
$439k
NIAID NIH HHS R21 AI067971
6 · The paper itself

Abstract

Recent studies have shown that there are multiple clinically important members of the Aspergillus section Fumigati that are difficult to distinguish on the basis of morphological features (e.g., Aspergillus fumigatus, A. lentulus, and Neosartorya udagawae). Identification of these organisms may be clinically important, as some species vary in their susceptibilities to antifungal agents. In a prior study, we utilized multilocus sequence typing to describe A. lentulus as a species distinct from A. fumigatus. The sequence data show that the gene encoding beta-tubulin, benA, has high interspecies variability at intronic regions but is conserved among isolates of the same species. These data were used to develop a PCR-restriction fragment length polymorphism (PCR-RFLP) method that rapidly and accurately distinguishes A. fumigatus, A. lentulus, and N. udagawae, three major species within the section Fumigati that have previously been implicated in disease. Digestion of the benA amplicon with BccI generated unique banding patterns; the results were validated by screening a collection of clinical strains and by in silico analysis of the benA sequences of Aspergillus spp. deposited in the GenBank database. PCR-RFLP of benA is a simple method for the identification of clinically important, similar morphotypes of Aspergillus spp. within the section Fumigati.

Indexed as

Polymorphism, Restriction Fragment LengthAspergillusBase SequenceDeoxyribonucleases, Type II Site-SpecificDNA, FungalFungal ProteinsHumansMolecular Sequence DataMycological Typing TechniquesPolymerase Chain ReactionTubulinDeoxyribonucleases, Type II Site-SpecificDNA, Fungalendodeoxyribonuclease BccIFungal ProteinsTubulin

Identifiers

PMID19403766
PMCPMC2708504

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.