ArticleJournal of clinical microbiology2009
Rapid real-time nucleic Acid sequence-based amplification-molecular beacon platform to detect fungal and bacterial bloodstream infections.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 79 citations in OpenAlex.
- Complex In Silico RNA Design with MoiRNAiFold.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Isothermal Amplification of Nucleic Acids Coupled with Nanotechnology and Microfluidic Platforms for Detecting Antimicrobial Drug Resistance and Beyond.Advanced pharmaceutical bulletin · 2022Review
- Application and evaluation of nucleic acid sequence-based amplification, PCR and cryptococcal antigen test for diagnosis of cryptococcosis.BMC infectious diseases · 2021Article
- Mobile nucleic acid amplification testing (mobiNAAT) for Chlamydia trachomatis screening in hospital emergency department settings.Scientific reports · 2017Article
- Washed Away; How Not to Lose Your RNA during Isolation.Journal of biomolecular techniques : JBT · 2017Article
- Development and evaluation of enzyme-linked immunosorbent assay of nucleic acid sequence-based amplification for diagnosis of invasive aspergillosis.AMB Express · 2016Article
- Molecular Diagnostic Testing for Aspergillus.Journal of clinical microbiology · 2016Review
- Occurrence of Fungal DNA Contamination in PCR Reagents: Approaches to Control and Decontamination.Journal of clinical microbiology · 2016Article
- Rapid identification of medically important Candida isolates using high resolution melting analysis.PloS one · 2015Article
- Detection of multiple fungal species in blood samples by real-time PCR: an interpretative challenge.Journal of clinical microbiology · 2014Article
- Fungal DNA detected in blood samples of patients who received contaminated methylprednisolone injections reveals increased complexity of causative agents.Journal of clinical microbiology · 2014Article
- Article
- Single quantum dot analysis enables multiplexed point mutation detection by gap ligase chain reaction.Small (Weinheim an der Bergstrasse, Germany) · 2013Article
- Polymerase chain reaction-based assays for the diagnosis of invasive fungal infections.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2012Review
- Use and evaluation of molecular diagnostics for pneumonia etiology studies.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2012Review
- Real-time nucleic acid sequence-based amplification assay for rapid detection and quantification of agr functionality in clinical Staphylococcus aureus isolates.Journal of clinical microbiology · 2012Article
- Molecular beacons in diagnostics.F1000 medicine reports · 2012Article
- Article
- Article
- Molecular detection of bacterial pathogens using microparticle enhanced double-stranded DNA probes.Analytical chemistry · 2011Article
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Bloodstream infections (BSIs) are a significant cause of morbidity and mortality. Successful patient outcomes are diminished by a failure to rapidly diagnose these infections and initiate appropriate therapy. A rapid and reliable diagnostic platform of high sensitivity is needed for the management of patients with BSIs. The combination of an RNA-dependent nucleic acid sequence-based amplification and molecular beacon (NASBA-MB) detection system in multiplex format was developed to rapidly detect medically important BSI organisms. Probes and primers representing pan-gram-negative, pan-gram-positive, pan-fungal, pan-Candida, and pan-Aspergillus organisms were established utilizing 16S and 28S rRNA targets for bacteria and fungi, respectively. Two multiplex panels were developed to rapidly discriminate bacterial or fungal infections at the subkingdom/genus level with a sensitivity of 1 to 50 genomes. A clinical study was performed to evaluate the accuracy of this platform by evaluating 570 clinical samples from a tertiary-care hospital group using blood bottle samples. The sensitivity, specificity, and Youden's index values for pan-gram-positive detection and pan-gram-negative detection were 99.7%, 100%, 0.997 and 98.6%, 95.9%, 0.945, respectively. The positive predictive values (PPV) and the negative predictive values (NPV) for these two probes were 100, 90.7, and 99.4, 99.4, respectively. Pan-fungal and pan-Candida probes showed 100% sensitivity, specificity, PPV, and NPV, and the pan-Aspergillus probe showed 100% NPV. Robust signals were observed for all probes in the multiplex panels, with signal detection in <15 min. The multiplex real-time NASBA-MB assay provides a valuable platform for the rapid and specific diagnosis of bloodstream pathogens, and reliable pathogen identification and characterization can be obtained in under 3 h.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.