ArticlePlant physiology2009
Two alternatively spliced isoforms of the Arabidopsis SR45 protein have distinct roles during normal plant development.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers.
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Who cites it
93 citing papers in PubMed.
- Arabidopsis Heterotrimeric G Beta Variants Shape Plant Development and Modulate Responses to Endoplasmic Reticulum Stress and Salt Stress.Plant, cell & environment · 2026Article
- Alternative splicing-dependent T218 phosphoregulation controls SR45.1 nuclear organization and salt stress responses in Arabidopsis.Plant cell reports · 2026Article
- Splicing regulation by RS2Z36 controls ovary patterning and fruit growth in tomato.Plant molecular biology · 2026Article
- Phosphate starvation induces root cell-type-specific transcriptional responses and alternative splicing.The New phytologist · 2026Article
- Differential splicing fine-tunes guard cell gene expression and is required for drought tolerance in Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2026Article
- Genome-Wide Isoform Switching Reveals SR45-Mediated Splicing Control ofInternational journal of molecular sciences · 2025Article
- Next-Generation Mapping of the ACINUS-Mediated Alternative Splicing Machinery and Its Regulation by O-glycosylation inbioRxiv : the preprint server for biology · 2025Article
- Article
- Nucleo-cytoplasmic distribution of SAP18 reveals its dual function in splicing regulation and heat-stress response in Arabidopsis.Plant communications · 2025Article
- A splicing regulator, SR45, suppresses plant immunity by regulating salicylic acid pathway inFrontiers in plant science · 2025Article
- Alternative splicing regulation in plants by SP7-like effectors from symbiotic arbuscular mycorrhizal fungi.Nature communications · 2024Article
- Membralin is required for maize development and defines a branch of the endoplasmic reticulum-associated degradation pathway in plants.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Mapping protein-RNA binding in plants with individual-nucleotide-resolution UV cross-linking and immunoprecipitation (plant iCLIP2).Nature protocols · 2024Review
- SCR106 splicing factor modulates abiotic stress responses by maintaining RNA splicing in rice.Journal of experimental botany · 2024Article
- Alternative splicing responses to salt stress inFrontiers in genetics · 2024Article
- A new mechanism of flowering regulation by the competition of isoforms in Osmanthus fragrans.Annals of botany · 2023Article
- The Splicing Factor SR45 Negatively Regulates Anthocyanin Accumulation under High-Light Stress inLife (Basel, Switzerland) · 2023Article
- Toward a systems view on RNA-binding proteins and associated RNAs in plants: Guilt by association.The Plant cell · 2023Article
- Catsnap: a user-friendly algorithm for determining the conservation of protein variants reveals extensive parallelisms in the evolution of alternative splicing.The New phytologist · 2023Article
- TwoPlants (Basel, Switzerland) · 2023Article
33 more citing papers are in PubMed but not listed here.
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2 authors.
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Abstract
The serine-arginine-rich (SR) proteins constitute a conserved family of pre-mRNA splicing factors. In Arabidopsis (Arabidopsis thaliana), they are encoded by 19 genes, most of which are themselves alternatively spliced. In the case of SR45, the use of alternative 3' splice sites 21 nucleotides apart generates two alternatively spliced isoforms. Isoform 1 (SR45.1) has an insertion relative to isoform 2 (SR45.2) that replaces a single arginine with eight amino acids (TSPQRKTG). The biological implications of SR45 alternative splicing have been unclear. A previously described loss-of-function mutant affecting both isoforms, sr45-1, shows several developmental defects, including defects in petal development and root growth. We found that the SR45 promoter is highly active in regions with actively growing and dividing cells. We also tested the ability of each SR45 isoform to complement the sr45-1 mutant by overexpression of isoform-specific green fluorescent protein (GFP) fusion proteins. As expected, transgenic plants overexpressing either isoform displayed both nuclear speckles and GFP fluorescence throughout the nucleoplasm. We found that SR45.1-GFP complements the flower petal phenotype, but not the root growth phenotype. Conversely, SR45.2-GFP complements root growth but not floral morphology. Mutation of a predicted phosphorylation site within the alternatively spliced segment, SR45.1-S219A-GFP, does not affect complementation. However, a double mutation affecting both serine-219 and the adjacent threonine-218 (SR45.1-T218A + S219A-GFP) behaves like isoform 2, complementing the root but not the floral phenotype. In conclusion, our study provides evidence that the two alternatively spliced isoforms of SR45 have distinct biological functions.
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