ArticleMolecular biology of the cell2009
The class V myosin Myo2p is required for Fus2p transport and actin polarization during the yeast mating response.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Two- and Three-Dimensional Tracking ofCells · 2020Article
- Membrane curvature directs the localization of Cdc42p to novel foci required for cell-cell fusion.The Journal of cell biology · 2017Article
- Molecular basis of Kar9-Bim1 complex function during mating and spindle positioning.Molecular biology of the cell · 2016Article
- Article
- Identifying novel protein phenotype annotations by hybridizing protein-protein interactions and protein sequence similarities.Molecular genetics and genomics : MGG · 2016Article
- An Amphiphysin-Like Domain in Fus2p Is Required for Rvs161p Interaction and Cortical Localization.G3 (Bethesda, Md.) · 2015Article
- Computational model of polarized actin cables and cytokinetic actin ring formation in budding yeast.Cytoskeleton (Hoboken, N.J.) · 2015Article
- A formin-nucleated actin aster concentrates cell wall hydrolases for cell fusion in fission yeast.The Journal of cell biology · 2015Article
- To avoid a mating mishap, yeast focus and communicate.The Journal of cell biology · 2015Article
- Article
- Mate and fuse: how yeast cells do it.Open biology · 2013Review
- A mechanism for the coordination of proliferation and differentiation by spatial regulation of Fus2p in budding yeast.Genes & development · 2012Article
- Cdc42p and Fus2p act together late in yeast cell fusion.Molecular biology of the cell · 2012Article
- Prm1 targeting to contact sites enhances fusion during mating in Saccharomyces cerevisiae.Eukaryotic cell · 2010Article
- Polarization of the yeast pheromone receptor requires its internalization but not actin-dependent secretion.Molecular biology of the cell · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Mating yeast cells remove their cell walls and fuse their plasma membranes in a spatially restricted cell contact region. Cell wall removal is dependent on Fus2p, an amphiphysin-associated Rho-GEF homolog. As mating cells polarize, Fus2p-GFP localizes to the tip of the mating projection, where cell fusion will occur, and to cytoplasmic puncta, which show rapid movement toward the tip. Movement requires polymerized actin, whereas tip localization is dependent on both actin and a membrane protein, Fus1p. Here, we show that Fus2p-GFP movement is specifically dependent on Myo2p, a type V myosin, and not on Myo4p, another type V myosin, or Myo3p and Myo5p, type I myosins. Fus2p-GFP tip localization and actin polarization in shmoos are also dependent on Myo2p. A temperature-sensitive tropomyosin mutation and Myo2p alleles that specifically disrupt vesicle binding caused rapid loss of actin patch organization, indicating that transport is required to maintain actin polarity. Mutant shmoos lost actin polarity more rapidly than mitotic cells, suggesting that the maintenance of cell polarity in shmoos is more sensitive to perturbation. The different velocities, differential sensitivity to mutation and lack of colocalization suggest that Fus2p and Sec4p, another Myo2p cargo associated with exocytotic vesicles, reside predominantly on different cellular organelles.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.