ArticleMolecular biology of the cell2009
Dynein-driven mitotic spindle positioning restricted to anaphase by She1p inhibition of dynactin recruitment.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 45 citations in OpenAlex.
- Loss of symmetric cell division of apical neural progenitors drives DENND5A-related developmental and epileptic encephalopathy.Nature communications · 2024Article
- Loss of symmetric cell division of apical neural progenitors drivesmedRxiv : the preprint server for health sciences · 2024Article
- The microtubule-associated protein She1 coordinates directional spindle positioning by spatially restricting dynein activity.Journal of cell science · 2021Article
- Modeling a disease-correlated tubulin mutation in budding yeast reveals insight into MAP-mediated dynein function.Molecular biology of the cell · 2021Article
- Help or hindrance: how do microtubule-based forces contribute to genome damage and repair?Current genetics · 2020Review
- Astral microtubule forces alter nuclear organization and inhibit DNA repair in budding yeast.Molecular biology of the cell · 2019Article
- Selective defects in gene expression control genome instability in yeast splicing mutants.Molecular biology of the cell · 2019Article
- Nuclear movement in fungi.Seminars in cell & developmental biology · 2018Review
- She1 affects dynein through direct interactions with the microtubule and the dynein microtubule-binding domain.Nature communications · 2017Article
- Microtubule cross-linking activity of She1 ensures spindle stability for spindle positioning.The Journal of cell biology · 2017Article
- Mechanism of nuclear movements in a multinucleated cell.Molecular biology of the cell · 2017Article
- Regulation of mitotic spindle orientation: an integrated view.EMBO reports · 2016Review
- The dynein cortical anchor Num1 activates dynein motility by relieving Pac1/LIS1-mediated inhibition.The Journal of cell biology · 2015Article
- Cytokinesis breaks dicentric chromosomes preferentially at pericentromeric regions and telomere fusions.Genes & development · 2015Article
- Regulation of mitotic spindle disassembly by an environmental stress-sensing pathway in budding yeast.Genetics · 2014Article
- Stopped in its tracks: negative regulation of the dynein motor by the yeast protein She1.BioEssays : news and reviews in molecular, cellular and developmental biology · 2013Article
- Mechanisms of spindle positioning.The Journal of cell biology · 2013Review
- She1-mediated inhibition of dynein motility along astral microtubules promotes polarized spindle movements.Current biology : CB · 2012Article
- The yeast homologue of the microtubule-associated protein Lis1 interacts with the sumoylation machinery and a SUMO-targeted ubiquitin ligase.Molecular biology of the cell · 2012Article
- BICD2, dynactin, and LIS1 cooperate in regulating dynein recruitment to cellular structures.Molecular biology of the cell · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Dynein is a minus-end-directed microtubule motor important for mitotic spindle positioning. In budding yeast, dynein activity is restricted to anaphase when the nucleus enters the bud neck, yet the nature of the underlying regulatory mechanism is not known. Here, the microtubule-associated protein She1p is identified as a novel regulator of dynein activity. In she1 Delta cells, dynein is activated throughout the cell cycle, resulting in aberrant spindle movements that misposition the spindle. We also found that dynactin, a cofactor essential for dynein motor function, is a dynamic complex whose recruitment to astral microtubules (aMTs) increases dramatically during anaphase. Interestingly, loss of She1p eliminates the cell-cycle regulation of dynactin recruitment and permits enhanced dynactin accumulation on aMTs throughout the cell cycle. Furthermore, localization of the dynactin complex to aMTs requires dynein, suggesting that dynactin is recruited to aMTs via interaction with dynein and not the microtubule itself. Lastly, we present evidence supporting the existence of an incomplete dynactin subcomplex localized at the SPB, and a complete complex that is loaded onto aMTs from the cytoplasm. We propose that She1p restricts dynein-dependent spindle positioning to anaphase by inhibiting the association of dynein with the complete dynactin complex.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.