ArticleJournal of virology2009
Theiler's murine encephalomyelitis virus leader protein is the only nonstructural protein tested that induces apoptosis when transfected into mammalian cells.
Article in Journal of virology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- MicroRNA-detargeting proves more effective thanMolecular therapy oncolytics · 2021Article
- The Leader Protein of Theiler's Virus Prevents the Activation of PKR.Journal of virology · 2019Article
- The Saffold Virus-Penang 2B and 3C Proteins, but not the L Protein, Induce Apoptosis in HEp-2 and Vero Cells.Virologica Sinica · 2019Article
- Intracellular localization of Saffold virus Leader (L) protein differs in Vero and HEp-2 cells.Emerging microbes & infections · 2016Article
- Saffold virus is able to productively infect primate and rodent cell lines and induces apoptosis in these cells.Emerging microbes & infections · 2014Article
- Mutation of the Theiler's virus leader protein zinc-finger domain impairs apoptotic activity in murine macrophages.Virus research · 2013Article
- Suppression of injuries caused by a lytic RNA virus (mengovirus) and their uncoupling from viral reproduction by mutual cell/virus disarmament.Journal of virology · 2012Article
- Saffold virus, a novel human Cardiovirus with unknown pathogenicity.Journal of virology · 2012Review
- Encephalomyocarditis virus 2A protein is required for viral pathogenesis and inhibition of apoptosis.Journal of virology · 2011Article
- The L-coding region of the DA strain of Theiler's murine encephalomyelitis virus causes dysfunction and death of myelin-synthesizing cells.Journal of virology · 2011Article
- The leader protein of cardioviruses inhibits stress granule assembly.Journal of virology · 2011Article
- Apoptotic and antiapoptotic activity of L protein of Theiler's murine encephalomyelitis virus.Journal of virology · 2011Article
- Theiler's virus infection: Pathophysiology of demyelination and neurodegeneration.Pathophysiology : the official journal of the International Society for Pathophysiology · 2011Article
- Viral security proteins: counteracting host defences.Nature reviews. Microbiology · 2010Review
- Different strains of Theiler's murine encephalomyelitis virus antagonize different sites in the type I interferon pathway.Journal of virology · 2010Article
- Cultivation and serological characterization of a human Theiler's-like cardiovirus associated with diarrheal disease.Journal of virology · 2010Article
- Cytokine/chemokine profile in J774 macrophage cells persistently infected with DA strain of Theiler's murine encephalomyelitis virus (TMEV).Journal of neurovirology · 2010Article
- Theiler's murine encephalomyelitis virus L* amino acid position 93 is important for virus persistence and virus-induced demyelination.Journal of virology · 2010Article
- Activation of tumor suppressor protein p53 is required for Theiler's murine encephalomyelitis virus-induced apoptosis in M1-D macrophages.Journal of virology · 2009Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Theiler's murine encephalomyelitis virus (TMEV) induces two distinct cell death programs, necrosis and apoptosis. The apoptotic pathway is of particular interest because TMEV persists in the central nervous system of mice, largely in infiltrating macrophages, which undergo apoptosis. Infection of murine macrophages in culture induces apoptosis that is Bax dependent through the intrinsic or mitochondrial pathway, restricting infectious-virus yields and raising the possibility that apoptosis represents a mechanism to attenuate TMEV yet promote macrophage-to-macrophage spread during persistent infection. To help define the cellular stressors and upstream signaling events leading to apoptosis during TMEV infection, we screened baby hamster kidney (BHK-21) cells transfected to express individual nonstructural genes (except 3B) of the low-neurovirulence BeAn virus strain for cell death. Only expression of the leader protein led to apoptosis, as assessed by fluorescence-activated cell sorting analysis of propidium iodide- and annexin V-stained transfected cells, immunoblot analysis of poly(ADP-ribose) polymerase and caspase cleavages, electron microscopy, and inhibition of apoptosis by the pancaspase inhibitor qVD-OPh. After transfection, Bak and not Bax expression increased, suggesting that the apical pathway leading to activation of these Bcl-2 multi-BH-domain proapoptotic proteins differs in BeAn virus infection versus L transfection. Mutation to remove the CHCC Zn finger motif from L, a motif required by L to mediate inhibition of nucleocytoplasmic trafficking, significantly reduced L-protein-induced apoptosis in both BHK-21 and M1-D macrophages.
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