ArticleThe Journal of biological chemistry2009
A unique 2-sulfated {beta}-galactan from the egg jelly of the sea urchin Glyptocidaris crenularis: conformation flexibility versus induction of the sperm acrosome reaction.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 48 citations in OpenAlex.
- Structural and functional insights into sulfated galactans: a systematic review.Glycoconjugate journal · 2010Pooled it
- Chromosome-Level Genome Assembly of the Sea Urchin Glyptocidaris crenularis and Sex Chromosome Identification.Biochemical genetics · 2026Article
- Marine sulfated glycans inhibit the interaction of heparin with S-protein of SARS-CoV-2 Omicron XBB variant.Glycoconjugate journal · 2024Article
- Structure and Binding Properties to Blood Co-Factors of the Least Sulfated Galactan Found in the Cell Wall of the Red AlgaMarine drugs · 2024Article
- Direct antitumoral effects of sulfated fucans isolated from echinoderms: a possible role of neuropilin-1/β1 integrin endocytosis and focal adhesion kinase degradation.Glycobiology · 2023Article
- Structure, anti-SARS-CoV-2, and anticoagulant effects of two sulfated galactans from the red alga Botryocladia occidentalis.International journal of biological macromolecules · 2023Article
- SPR Sensor-Based Analysis of the Inhibition of Marine Sulfated Glycans on Interactions between Monkeypox Virus Proteins and Glycosaminoglycans.Marine drugs · 2023Article
- Fractionation of sulfated galactan from the red alga Botryocladia occidentalis separates its anticoagulant and anti-SARS-CoV-2 properties.The Journal of biological chemistry · 2022Article
- Red Algal Sulfated Galactan Binds and Protects Neural Cells from HIV-1 gp120 and Tat.Pharmaceuticals (Basel, Switzerland) · 2021Article
- The Sea as a Rich Source of Structurally Unique Glycosaminoglycans and Mimetics.Microorganisms · 2017Review
- Structural and haemostatic features of pharmaceutical heparins from different animal sources: challenges to define thresholds separating distinct drugs.Scientific reports · 2016Article
- Perspective on the use of sulfated polysaccharides from marine organisms as a source of new antithrombotic drugs.Marine drugs · 2015Review
- Sulfated glycans in sea urchin fertilization.Glycoconjugate journal · 2015Review
- Conformational characterization of ipomotaosides and their recognition by COX-1 and 2.Molecules (Basel, Switzerland) · 2014Article
- Specific sulfation and glycosylation-a structural combination for the anticoagulation of marine carbohydrates.Frontiers in cellular and infection microbiology · 2014Review
- Bovine and porcine heparins: different drugs with similar effects on human haemodialysis.BMC research notes · 2013Article
- Carbohydrate force fields.Wiley interdisciplinary reviews. Computational molecular science · 2012Article
- Fucanomics and galactanomics: marine distribution, medicinal impact, conceptions, and challenges.Marine drugs · 2012Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sulfated polysaccharides from the egg jelly of sea urchins act as species-specific inducers of the sperm acrosome reaction, which is a rare molecular mechanism of carbohydrate-induced signal-transduction event in animal cells. The sea urchin polysaccharides differ in monosaccharide composition (l-fucose or l-galactose), glycosylation, and sulfation sites, but they are always in the alpha-anomeric configuration. Herein, structural analysis of the polysaccharide from the sea urchin Glyptocidaris crenularis surprisingly revealed a unique sulfated beta-d-galactan composed by (3-beta-d-Galp-2(OSO(3))-1-->3-beta-d-Galp-1)(n) repeating units. Subsequently, we used the G. crenularis galactan to compare different 2-sulfated polysaccharides as inducers of the acrosome reaction using homologous and heterologous sperm. We also tested the effect of chemically over-sulfated galactans. Intriguingly, the anomeric configuration of the glycosidic linkage rather than the monosaccharide composition (galactose or fucose) is the preferential structural requirement for the effect of these polysaccharides on sea urchin fertilization. Nuclear magnetic resonance and molecular dynamics indicate that sulfated alpha-galactan or alpha-fucan have less dynamic structural behavior, exhibiting fewer conformational populations, with an almost exclusive conformational state with glycosidic dihedral angles Phi/Psi = -102 degrees /131 degrees . The preponderant conformer observed in the sulfated alpha-galactan or alpha-fucan is not observed among populations in the beta-form despite its more flexible structure in solution. Possibly, a proper spatial arrangement is required for interaction of the sea urchin-sulfated polysaccharides with the specific sperm receptor.
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Registered trials
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