ArticleAnalytical chemistry2009
Quantification of heparan sulfate disaccharides using ion-pairing reversed-phase microflow high-performance liquid chromatography with electrospray ionization trap mass spectrometry.
Article in Analytical chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed, 91 citations in OpenAlex.
- Investigation of sample handling steps for accurate heparan sulphate disaccharide analysis using HPLC-MS.Electrophoresis · 2025Article
- UtilizingAmerican journal of physiology. Cell physiology · 2025Review
- Article
- [Advances in heparin structural analysis by chromatography technologies].Se pu = Chinese journal of chromatography · 2023Review
- Heparinase Digestion of 3-Frontiers in medicine · 2022Article
- Stability and recovery issues concerning chondroitin sulfate disaccharide analysis.Analytical and bioanalytical chemistry · 2021Article
- Investigation of the biological functions of heparan sulfate using a chemoenzymatic synthetic approach.RSC chemical biology · 2021Review
- Review
- A Glycosaminoglycan Extract fromMarine drugs · 2019Article
- Analysis of sulfates on low molecular weight heparin using mass spectrometry: structural characterization of enoxaparin.Expert review of proteomics · 2018Review
- Sensitive method for glycosaminoglycan analysis of tissue sections.Journal of chromatography. A · 2018Article
- Capillary electrophoresis for total glycosaminoglycan analysis.Analytical and bioanalytical chemistry · 2014Article
- Structure and activity of a new low-molecular-weight heparin produced by enzymatic ultrafiltration.Journal of pharmaceutical sciences · 2014Article
- Analysis of glycosaminoglycan-derived, precolumn, 2-aminoacridone-labeled disaccharides with LC-fluorescence and LC-MS detection.Nature protocols · 2014Article
- FGF-FGFR signaling mediated through glycosaminoglycans in microtiter plate and cell-based microarray platforms.Biochemistry · 2013Article
- Immobilized enzymes to convert N-sulfo, N-acetyl heparosan to a critical intermediate in the production of bioengineered heparin.Journal of biotechnology · 2013Article
- Structural characterization of pharmaceutical heparins prepared from different animal tissues.Journal of pharmaceutical sciences · 2013Article
- Glycosaminoglycan glycomics using mass spectrometry.Molecular & cellular proteomics : MCP · 2013Review
- Top-down approach for the direct characterization of low molecular weight heparins using LC-FT-MS.Analytical chemistry · 2012Article
- Metabolic engineering of Chinese hamster ovary cells: towards a bioengineered heparin.Metabolic engineering · 2012Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The glycosaminoglycan (GAG) family of biomacromolecules is composed acidic and linear chains of repeating disaccharide units. Quantitative disaccharide composition analysis is essential for the study and characterization of GAGs. Heparan sulfate and heparin consist of multiple disaccharide units and can be well-separated by ion-pairing reversed-phase microflow high-performance liquid chromatography (IPRP-Mf-HPLC). Each disaccharide can be detected and its mass confirmed by electrospray ionization mass spectrometry (ESI-MS). Isotopically enriched disaccharides were prepared chemoenzymatically from a uniformly (13)C,(15)N-labeled N-acetylheparosan (-GlcA(1-->4)GlcNAc-) obtained from the fermentation of E. coli K5. These isotopically enriched disaccharides have identical HPLC retention times and mass spectra as their unlabeled counterparts and were used in liquid chromatography-mass spectrometry (LC-MS) as internal standards. The ratio of intensities between each pair of enriched and nonenriched disaccharides showed a linear relationship as a function of concentration. With the use of these calibration curves, the amount of each disaccharide (> or = 2 ng/disaccharide) could be quantified in four heparan sulfate samples analyzed by this method.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.