ArticlePloS one2009
Both strands of siRNA have potential to guide posttranscriptional gene silencing in mammalian cells.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 41 citations in OpenAlex.
- Small Interfering RNA (siRNA) as a Targeted Therapy for Acute Respiratory Distress Syndrome: Evidence from Experimental Models.International journal of molecular sciences · 2026Review
- Specificity of oligonucleotide gene therapy (OGT) agents.Theranostics · 2022Review
- Chemical strategies for strand selection in short-interfering RNAs.RSC advances · 2021Review
- A systematic study on the influence of thermodynamic asymmetry of 5'-ends of siRNA duplexes in relation to their silencing potency.Scientific reports · 2019Article
- shRNA target prediction informed by comprehensive enquiry (SPICE): a supporting system for high-throughput screening of shRNA library.EURASIP journal on bioinformatics & systems biology · 2016Article
- Inhibition of Respiratory Syncytial Virus Replication by Simultaneous Targeting of mRNA and Genomic RNA Using Dual-Targeting siRNAs.Molecular biotechnology · 2016Article
- Blocking sense-strand activity improves potency, safety and specificity of anti-hepatitis B virus short hairpin RNA.EMBO molecular medicine · 2016Article
- Transcriptional gene silencing in humans.Nucleic acids research · 2016Article
- Furin Cleavage of L2 during Papillomavirus Infection: Minimal Dependence on Cyclophilins.Journal of virology · 2016Article
- Modulation of the RNA Interference Activity Using Central Mismatched siRNAs and Acyclic Threoninol Nucleic Acids (aTNA) Units.Molecules (Basel, Switzerland) · 2015Article
- Dicer-independent processing of short hairpin RNAs.Nucleic acids research · 2013Article
- Long non-coding RNA targeting and transcriptional de-repression.Nucleic acid therapeutics · 2013Review
- Synthesis, physicochemical and biological properties of oligonucleotides incorporated with amino-isonucleosides.Science China. Chemistry · 2012Article
- Development of Therapeutic-Grade Small Interfering RNAs by Chemical Engineering.Frontiers in genetics · 2012Article
- Modified siRNA structure with a single nucleotide bulge overcomes conventional siRNA-mediated off-target silencing.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Article
- The emerging role of RNA in the regulation of gene transcription in human cells.Seminars in cell & developmental biology · 2011Review
- Improved siRNA/shRNA functionality by mismatched duplex.PloS one · 2011Article
- Differential genomic imprinting and expression of imprinted microRNAs in testes-derived male germ-line stem cells in mouse.PloS one · 2011Article
- What are natural antisense transcripts good for?Biochemical Society transactions · 2010Review
- Novel RNA-based strategies for therapeutic gene silencing.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite the widespread application of RNA interference (RNAi) as a research tool for diverse purposes, the key step of strand selection of siRNAs during the formation of RNA-induced silencing complex (RISC) remains poorly understood. Here, using siRNAs targeted to the complementary region of Survivin and the effector protease receptor 1 (EPR-1), we show that both strands of the siRNA duplex can find their target mRNA and are equally eligible for assembly into Argonaute 2 (Ago2) of RISC in HEK293 cells. Transfection of the synthetic siRNA duplexes with different thermodynamic profiles or short hairpin RNA (shRNA) vectors that generate double-stranded RNAs (dsRNAs), permitting processing specifically from either the 5' or 3' end of the incipient siRNA, results in the degradation of the respective target mRNAs of either strand of the siRNA duplex with comparable efficiencies. Thus, while most RNAi reactions may follow the thermodynamic asymmetry rule in strand selection, our study suggests an exceptional mode for certain siRNAs in which both strands of the duplex are competent in sponsoring RNAi, and implies additional factors that might dictate the RNAi targets.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.