ArticleJournal of cell science2009
A novel mode of cell detachment from fibrillar fibronectin matrix under shear.
Article in Journal of cell science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Progressive endothelial coverage enhances hemocompatibility and prevents calcification in bioprosthetic valve tissue.Angiogenesis · 2026Article
- Differential Regulation of Neurite Outgrowth and Growth Cone Morphology by 3D Fibronectin and Fibronectin-Collagen Extracellular Matrices.Molecular neurobiology · 2022Article
- EGFRvIII uses intrinsic and extrinsic mechanisms to reduce glioma adhesion and increase migration.Journal of cell science · 2020Article
- Cell-derived decellularized extracellular matrices.Methods in cell biology · 2018Article
- Development of hybrid scaffolds with natural extracellular matrix deposited within synthetic polymeric fibers.Journal of biomedical materials research. Part A · 2017Article
- Nerve Guidance by a Decellularized Fibroblast Extracellular Matrix.Matrix biology : journal of the International Society for Matrix Biology · 2017Article
- Expansion of bone marrow-derived human mesenchymal stem/stromal cells (hMSCs) using a two-phase liquid/liquid system.Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986) · 2017Article
- Stimulatory effects of advanced glycation endproducts (AGEs) on fibronectin matrix assembly.Matrix biology : journal of the International Society for Matrix Biology · 2017Article
- Modulating wall shear stress gradient via equilateral triangular channel forBiomicrofluidics · 2016Article
- The cytoskeleton regulates cell attachment strength.Biophysical journal · 2015Article
- Measurement systems for cell adhesive forces.Journal of biomechanical engineering · 2015Article
- Article
- The role of filopodia in the recognition of nanotopographies.Scientific reports · 2013Article
- Fibronectin extra domain-A promotes hepatic stellate cell motility but not differentiation into myofibroblasts.Gastroenterology · 2012Article
- Fibronectins, their fibrillogenesis, and in vivo functions.Cold Spring Harbor perspectives in biology · 2011Review
- Stiffness gradients mimicking in vivo tissue variation regulate mesenchymal stem cell fate.PloS one · 2011Article
- Microelastic properties of lung cell-derived extracellular matrix.Acta biomaterialia · 2011Article
- Alpha5beta1 integrin-fibronectin interactions specify liquid to solid phase transition of 3D cellular aggregates.PloS one · 2010Article
- Physico-mechanical aspects of extracellular matrix influences on tumorigenic behaviors.Seminars in cancer biology · 2010Review
- Detecting cell-adhesive sites in extracellular matrix using force spectroscopy mapping.Journal of physics. Condensed matter : an Institute of Physics journal · 2010Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Cells within tissues are surrounded by fibrillar extracellular matrix (ECM) that supports cell adhesion via integrin receptors. The strength of cell interactions with fibrillar matrix and the effects of force on these interactions have not been quantified. To this end, we used a spinning disc device to apply radially increasing shear to human HT1080 fibrosarcoma cells attached to a cell-derived fibrillar fibronectin (FN) matrix. The shear required to detach 50% of HT1080 cells was eight times greater on a FN-coated, rigid glass substrate than on fibrillar FN matrix. Covalent crosslinking of the FN matrix increased its stiffness tenfold and produced a modest increase in shear detachment force for these cells. On FN-coated surfaces, cells detach by releasing interactions between alpha5beta1 integrin and FN. By contrast, cell detachment from fibrillar matrix occurred through a novel mechanism of fibril breakage, which left holes in the matrix visible by fluorescence microscopy. These results show that cells require less force to detach from fibrillar matrix than from FN adsorbed on glass and that detachment occurs through breaking fibrils instead of by release of integrin-matrix bonds. Thus, ECM fibril breakage is another molecular feature to consider when understanding cell and tissue homeostasis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.