ArticleMolecular plant pathology2009
Development of taxon-specific sequence characterized amplified region (SCAR) markers based on actin sequences and DNA amplification fingerprinting (DAF): a case study in the Phoma exigua species complex.
Article in Molecular plant pathology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Article
- Characterization of Boeremia exigua causing stem necrotic lesions on Luobuma in northwest China.Scientific reports · 2022Article
- Plastic-inhabiting fungi in marine environments and PCL degradation activity.Antonie van Leeuwenhoek · 2022Article
- The phoma-like dilemma.Studies in mycology · 2020Article
- Article
- Genera of phytopathogenic fungi: GOPHY 1.Studies in mycology · 2017Article
- Use of metabolomics for the chemotaxonomy of legume-associated Ascochyta and allied genera.Scientific reports · 2016Article
- Endophytic l-asparaginase-producing fungi from plants associated with anticancer properties.Journal of advanced research · 2015Article
- Resolving the Phoma enigma.Studies in mycology · 2015Article
- Advances in taxonomy of genus phoma: polyphyletic nature and role of phenotypic traits and molecular systematics.Indian journal of microbiology · 2014Review
- Species concepts in Cercospora: spotting the weeds among the roses.Studies in mycology · 2013Article
- Using the developmental gene bicoid to identify species of forensically important blowflies (Diptera: calliphoridae).BioMed research international · 2013Article
- Analysis of the mating-type loci of co-occurring and phylogenetically related species of Ascochyta and Phoma.Molecular plant pathology · 2012Article
- DNA barcoding of recently diverged species: relative performance of matching methods.PloS one · 2012Article
- Highlights of the Didymellaceae: A polyphasic approach to characterise Phoma and related pleosporalean genera.Studies in mycology · 2010Article
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6 authors.
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Abstract
Phoma exigua is considered to be an assemblage of at least nine varieties that are mainly distinguished on the basis of host specificity and pathogenicity. However, these varieties are also reported to be weak pathogens and secondary invaders on non-host tissue. In practice, it is difficult to distinguish P. exigua from its close relatives and to correctly identify isolates up to the variety level, because of their low genetic variation and high morphological similarity. Because of quarantine issues and phytosanitary measures, a robust DNA-based tool is required for accurate and rapid identification of the separate taxa in this species complex. The present study therefore aims to develop such a tool based on unique nucleotide sequence identifiers. More than 60 strains of P. exigua and related species were compared in terms of partial actin gene sequences, or analysed using DNA amplification fingerprinting (DAF) with short, arbitrary, mini-hairpin primers. Fragments in the fingerprint unique to a single taxon were identified, purified and sequenced. Alignment of the sequence data and subsequent primer trials led to the identification of taxon-specific sequence characterized amplified regions (SCARs), and to a set of specific oligonucleotide combinations that can be used to identify these organisms in plant quarantine inspections.
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