ArticleMolecular plant pathology2009
Characterization of a putative endoxylanase in the migratory plant-parasitic nematode Radopholus similis.
Article in Molecular plant pathology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 66 citations in OpenAlex.
- The SUbventral-Gland Regulator (SUGR-1) of nematode virulence.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Targeted transcriptomics reveals signatures of large-scale independent origins and concerted regulation of effector genes in Radopholus similis.PLoS pathogens · 2021Article
- Plant Parasitic Nematodes: A Review on Their Behaviour, Host Interaction, Management Approaches and Their Occurrence in Two Sites in the Republic of Ireland.Plants (Basel, Switzerland) · 2021Review
- A Venom Allergen-Like Protein, RsVAP, the First Discovered Effector Protein ofInternational journal of molecular sciences · 2021Article
- Current Insights into Migratory Endoparasitism: Deciphering the Biology, Parasitism Mechanisms, and Management Strategies of Key Migratory Endoparasitic Phytonematodes.Plants (Basel, Switzerland) · 2020Review
- Silencing ofInternational journal of molecular sciences · 2019Article
- Plant-parasitic nematodes respond to root exudate signals with host-specific gene expression patterns.PLoS pathogens · 2019Article
- The genome of the migratory nematode, Radopholus similis, reveals signatures of close association to the sedentary cyst nematodes.PloS one · 2019Article
- Genome Announcement: The Draft Genomes of TwoJournal of nematology · 2019Article
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- Genetically engineered bananas resistant to Xanthomonas wilt disease and nematodes.Food and energy security · 2017Review
- RNA Interference: A Novel Source of Resistance to Combat Plant Parasitic Nematodes.Frontiers in plant science · 2017Review
- Anatomical Alterations in Plant Tissues Induced by Plant-Parasitic Nematodes.Frontiers in plant science · 2017Review
- Influence of Bxpel1 Gene Silencing by dsRNA Interference on the Development and Pathogenicity of the Pine Wood Nematode, Bursaphelenchus xylophilus.International journal of molecular sciences · 2016Article
- Transcriptome Analysis of the Chrysanthemum Foliar Nematode, Aphelenchoides ritzemabosi (Aphelenchida: Aphelenchoididae).PloS one · 2016Article
- Genome wide comprehensive analysis and web resource development on cell wall degrading enzymes from phyto-parasitic nematodes.BMC plant biology · 2015Article
- Article
- Analysis of the transcriptome of Hirschmanniella oryzae to explore potential survival strategies and host-nematode interactions.Molecular plant pathology · 2014Article
- Top 10 plant-parasitic nematodes in molecular plant pathology.Molecular plant pathology · 2013Review
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant-parasitic nematodes have developed an arsenal of enzymes to degrade the rigid plant cell wall. In this article, we report the presence of a putative endoxylanase in the migratory endoparasitic nematode Radopholus similis. This enzyme is thought to facilitate the migration of the nematode, as it breaks down xylan, the major component of hemicellulose. The corresponding gene (Rs-xyl1) was cloned and the sequence revealed three small introns. Interestingly, the position of all three introns was conserved in a putative endoxylanase from Meloidogyne hapla, and the position of one intron was conserved in two endoxylanases from Meloidogyne incognita, which suggests a common ancestral gene. The spatial and temporal expression of the Rs-xyl1 gene was examined by in situ hybridization and semi-quantitative reverse transcriptase-polymerase chain reaction. The putative protein consists of a signal peptide, a catalytic domain and a carbohydrate-binding module (CBM). The catalytic domain showed similarity to both glycosyl hydrolase family 5 (GHF5) and GHF30 enzymes. Using Hidden Markov Model profiles and phylogenetic analysis, we were able to show that Rs-XYL1 and its closest homologues are not members of GHF5, as suggested previously, but rather form a subclass within GHF30. Silencing the putative endoxylanase by double-stranded RNA targeting of the CBM region resulted in an average decrease in infection of 60%, indicating that the gene is important for the nematode to complete its life cycle.
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