ArticleMolecular microbiology2009
Dissection of recognition determinants of Escherichia coli sigma32 suggests a composite -10 region with an 'extended -10' motif and a core -10 element.
Article in Molecular microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Differential association of primary and alternative σ factors with RNA polymerase of Mycobacterium tuberculosis during transcription elongation.Nucleic acids research · 2026Article
- Peering into the Bacterial Cell: From Transcription to Functional Genomics.Journal of molecular biology · 2025Review
- Mutations upstream fromJournal of bacteriology · 2024Article
- Structural Insight into the Mechanism of σ32-Mediated Transcription Initiation of Bacterial RNA Polymerase.Biomolecules · 2023Article
- Article
- Evaluating EcxR for Its Possible Role inInternational journal of molecular sciences · 2022Article
- Identification and Characterization of the Alternative σJournal of bacteriology · 2022Article
- Automated model-predictive design of synthetic promoters to control transcriptional profiles in bacteria.Nature communications · 2022Article
- Construction of Strong Promoters by Assembling Sigma Factor Binding Motifs.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Universal functions of the σ finger in alternative σ factors during transcription initiation by bacterial RNA polymerase.RNA biology · 2021Article
- Functional Characterization and Transcriptional Analysis of clpP of Xanthomonas campestris pv. campestris.Current microbiology · 2020Article
- Physiological effects of overexpressed sigma factors on fermentative stress response of Zymomonas mobilis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Sequence Determinants Spanning -10 Motif and Spacer Region Implicated in UniqueFrontiers in microbiology · 2019Article
- A novel short L-arginine responsive protein-coding gene (laoB) antiparallel overlapping to a CadC-like transcriptional regulator in Escherichia coli O157:H7 Sakai originated by overprinting.BMC evolutionary biology · 2018Article
- An essential regulatory function of the DnaK chaperone dictates the decision between proliferation and maintenance in Caulobacter crescentus.PLoS genetics · 2017Article
- Sequence determinants spanning -35 motif and AT-rich spacer region impacting Ehrlichia chaffeensis Sigma 70-dependent promoter activity of two differentially expressed p28 outer membrane protein genes.DNA research : an international journal for rapid publication of reports on genes and genomes · 2016Article
- Bacterial Sigma Factors and Anti-Sigma Factors: Structure, Function and Distribution.Biomolecules · 2015Review
- Genetic and phenotypic characterization of the heat shock response in Pseudomonas putida.MicrobiologyOpen · 2014Article
- Structural basis for -10 promoter element melting by environmentally induced sigma factors.Nature structural & molecular biology · 2014Article
- Heat shock transcription factor σ32 co-opts the signal recognition particle to regulate protein homeostasis in E. coli.PLoS biology · 2013Article
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Funding
Abstract
Sigma32 controls expression of heat shock genes in Escherichia coli and is widely distributed in proteobacteria. The distinguishing feature of sigma32 promoters is a long -10 region (CCCCATNT) whose tetra-C motif is important for promoter activity. Using alanine-scanning mutagenesis of sigma32 and in vivo and in vitro assays, we identified promoter recognition determinants of this motif. The most downstream C (-13) is part of the -10 motif; our work confirms and extends recognition determinants of -13C. Most importantly, our work suggests that the two upstream Cs (-16, -15) constitute an 'extended -10' recognition motif that is recognized by K130, a residue universally conserved in beta- and gamma-proteobacteria. This residue is located in the alpha-helix of sigmaDomain 3 that mediates recognition of the extended -10 promoter motif in other sigmas. K130 is not conserved in alpha- and delta-/epsilon-proteobacteria and we found that sigma32 from the alpha-proteobacterium Caulobacter crescentus does not need the extended -10 motif for high promoter activity. This result supports the idea that K130 mediates extended -10 recognition. Sigma32 is the first Group 3 sigma shown to use the 'extended -10' recognition motif.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.