ArticleMolecular microbiology2009
Mutational analysis of Escherichia coli sigma28 and its target promoters reveals recognition of a composite -10 region, comprised of an 'extended -10' motif and a core -10 element.
Article in Molecular microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed.
- Structural insights into σ28-dependent transcription initiation and its regulation by anti-sigma factor in Pseudomonas aeruginosa.Nucleic acids research · 2026Article
- Differential association of primary and alternative σ factors with RNA polymerase of Mycobacterium tuberculosis during transcription elongation.Nucleic acids research · 2026Article
- Article
- Peering into the Bacterial Cell: From Transcription to Functional Genomics.Journal of molecular biology · 2025Review
- Article
- Identification and Characterization of the Alternative σJournal of bacteriology · 2022Article
- DNA Input Classification by a Riboregulator-Based Cell-Free Perceptron.ACS synthetic biology · 2022Article
- Universal functions of the σ finger in alternative σ factors during transcription initiation by bacterial RNA polymerase.RNA biology · 2021Article
- Antibiotic tolerance is associated with a broad and complex transcriptional response in E. coli.Scientific reports · 2021Article
- Structural basis of bacterial σThe EMBO journal · 2020Article
- Promoter activity of ORF-less gene cassettes isolated from the oral metagenome.Scientific reports · 2019Article
- Structural basis of ECF-σ-factor-dependent transcription initiation.Nature communications · 2019Article
- The evolutionary impact of intragenic FliA promoters in proteobacteria.Molecular microbiology · 2018Article
- Expression of the phosphodiesterase BifA facilitating swimming motility is partly controlled by FliA in Pseudomonas putida KT2440.MicrobiologyOpen · 2017Article
- Article
- Bacterial Sigma Factors and Anti-Sigma Factors: Structure, Function and Distribution.Biomolecules · 2015Review
- MG428 is a novel positive regulator of recombination that triggers mgpB and mgpC gene variation in Mycoplasma genitalium.Molecular microbiology · 2014Article
- Structural basis for -10 promoter element melting by environmentally induced sigma factors.Nature structural & molecular biology · 2014Article
- Article
- DNA-binding properties of the Bacillus subtilis and Aeribacillus pallidus AC6 σ(D) proteins.Journal of bacteriology · 2011Article
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Abstract
Sigma28 controls the expression of flagella-related genes and is the most widely distributed alternative sigma factor, present in motile Gram-positive and Gram-negative bacteria. The distinguishing feature of sigma28 promoters is a long -10 region (GCCGATAA). Despite the fact that the upstream GC is highly conserved, previous studies have not indicated a functional role for this motif. Here we examine the functional relevance of the GCCG motif and determine which residues in sigma28 participate in its recognition. We find that the GCCG motif is a functionally important composite element. The upstream GC constitutes an extended -10 motif and is recognized by R91, a residue in Domain 3 of sigma28. The downstream CG is the upstream edge of -10 region of the promoter; two residues in Region 2.4, D81 and R84, participate in its recognition. Consistent with their role in base-specific recognition of the promoter, R91, D81 and D84 are universally conserved in sigma28 orthologues. Sigma28 is the second Group 3 sigma shown to use an extended -10 region in promoter recognition, raising the possibility that other Group 3 sigmas will do so as well.
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