ArticleMolecular microbiology2009
Two small ncRNAs jointly govern virulence and transmission in Legionella pneumophila.
Article in Molecular microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
88 citing papers in PubMed.
- A Nutrient-Responsive LuxR Regulator Orchestrates Effector Gene Expression Across the Legionella Genus.Molecular microbiology · 2026Article
- Host-Specific Adaptation of Legionella pneumophila to Single and Multiple Hosts.Molecular biology and evolution · 2025Article
- Article
- Transcription factors DksA and PsrA are synergistic contributors toMicrobiology (Reading, England) · 2025Article
- Small molecule communication ofMicrobiology and molecular biology reviews : MMBR · 2024Review
- Ecology ofBiofilm · 2024Review
- GacA reduces virulence and increases competitiveness in planta in the tumorigenic olive pathogenFrontiers in plant science · 2024Article
- Nucleotides as Bacterial Second Messengers.Molecules (Basel, Switzerland) · 2023Review
- Structural basis for the toxicity of Legionella pneumophila effector SidH.Nature communications · 2023Article
- Article
- Two-component system GacS/GacA, a global response regulator of bacterial physiological behaviors.Engineering microbiology · 2023Review
- Developing New Tools to Fight Human Pathogens: A Journey through the Advances in RNA Technologies.Microorganisms · 2022Review
- Translocated Legionella pneumophila small RNAs mimic eukaryotic microRNAs targeting the host immune response.Nature communications · 2022Article
- Pathogenicity and Virulence ofVirulence · 2021Review
- Amplifying and Fine-Tuning Rsm sRNAs Expression and Stability to Optimize the Survival ofMicroorganisms · 2021Article
- The Small Protein YmoA Controls the Csr System and Adjusts Expression of Virulence-Relevant Traits ofFrontiers in microbiology · 2021Article
- Interplay between Regulatory RNAs and Signal Transduction Systems during Bacterial Infection.Genes · 2020Review
- A NovelmBio · 2020Article
- A CsrA-Binding,Journal of bacteriology · 2019Article
- A Two-Component System That Modulates Cyclic di-GMP Metabolism Promotes Legionella pneumophila Differentiation and Viability in Low-Nutrient Conditions.Journal of bacteriology · 2019Article
28 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
To transit from intra- to extracellular environments, Legionella pneumophila differentiates from a replicative/non-virulent to a transmissive/virulent form using the two-component system LetA/LetS and the global repressor protein CsrA. While investigating how both regulators act co-ordinately we characterized two ncRNAs, RsmY and RsmZ, that link the LetA/LetS and CsrA regulatory networks. We demonstrate that LetA directly regulates their expression and show that RsmY and RsmZ are functional in Escherichia coli and are able to bind CsrA in vitro. Single mutants have no (ΔrsmY) or a little (ΔrsmZ) impact on virulence, but the ΔrsmYZ strain shows a drastic defect in intracellular growth in Acanthamoeba castellanii and THP-1 monocyte-derived macrophages. Analysis of the transcriptional programmes of the ΔletA, ΔletS and ΔrsmYZ strains revealed that the switch to the transmissive phase is partially blocked. One major difference between the ΔletA, ΔletS and ΔrsmYZ strains was that the latter synthesizes flagella. Taken together, LetA activates transcription of RsmY and RsmZ, which sequester CsrA and abolish its post-transcriptional repressive activity. However, the RsmYZ-CsrA pathway appears not to be the main or only regulatory circuit governing flagella synthesis. We suggest that rather RpoS and LetA, by influencing LetE and probably cyclic-di-GMP levels, regulate motility in L. pneumophila.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.