ReviewAnnual review of biomedical engineering2009
Fluorescent probes for live-cell RNA detection.
Review in Annual review of biomedical engineering, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
85 citing papers in PubMed.
- Detection of miR-133a-5p Using a Molecular Beacon Probe for Investigating Postmortem Intervals.Non-coding RNA · 2024Article
- Distinctive Nucleic Acid Recognition by Lysine-Embedded Phenanthridine Peptides.International journal of molecular sciences · 2024Article
- Inherent stochasticity, noise and limits of detection in continuous and time-gated fluorescence systems.PloS one · 2024Article
- Development of Highly Fluorogenic Styrene Probes for Visualizing RNA in Live Cells.ACS chemical biology · 2023Article
- Development of Fluorescent Turn-On Probes for CAG-RNA Repeats.Biosensors · 2022Article
- Development of a Highly Selective and Sensitive Fluorescent Probe for Imaging RNA Dynamics in Live Cells.Molecules (Basel, Switzerland) · 2022Article
- FISH-TAMB, a Fixation-Free mRNA Fluorescent Labeling Technique to Target Transcriptionally Active Members in Microbial Communities.Microbial ecology · 2022Article
- Illuminating RNA biology through imaging.Nature cell biology · 2022Review
- TRACE-seq: A transgenic system for unbiased and non-invasive transcriptome profiling of living cells.iScience · 2022Article
- DNA-enabled fluorescent-based nanosensors monitoring tumor-related RNA toward advanced cancer diagnosis: A review.Frontiers in bioengineering and biotechnology · 2022Review
- Advances of Exosomal miRNAs in Breast Cancer Progression and Diagnosis.Diagnostics (Basel, Switzerland) · 2021Review
- Article
- Recent Advances in the Molecular Beacon Technology for Live-Cell Single-Molecule Imaging.iScience · 2020Review
- Nucleic-Acid Structures as Intracellular Probes for Live Cells.Advanced materials (Deerfield Beach, Fla.) · 2020Review
- Nuclear and Cytoplasmatic Quantification of Unconjugated, Label-Free Locked Nucleic Acid Oligonucleotides.Nucleic acid therapeutics · 2020Article
- Fluorescence in situ hybridization (FISH) and cell sorting of living bacteria.Scientific reports · 2019Article
- CRISPR/dual-FRET molecular beacon for sensitive live-cell imaging of non-repetitive genomic loci.Nucleic acids research · 2019Article
- Multiplex live single-cell transcriptional analysis demarcates cellular functional heterogeneity.eLife · 2019Article
- Strategic labelling approaches for RNA single-molecule spectroscopy.RNA biology · 2019Review
- Simple alkanoyl acylating agents for reversible RNA functionalization and control.Chemical communications (Cambridge, England) · 2019Article
25 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Commonly used techniques for analyzing gene expression, such as polymerase chain reaction (PCR), microarrays, and in situ hybridization, have proven invaluable in understanding RNA processing and regulation. However, these techniques rely on the use of lysed and/or fixed cells and are therefore limited in their ability to provide important spatial-temporal information. This has led to the development of numerous techniques for imaging RNA in living cells, some of which have already provided important insight into the dynamic role RNA plays in dictating cell behavior. Here we review the fluorescent probes that have allowed for RNA imaging in living cells and discuss their utility and limitations. Common challenges faced by fluorescent probes, such as probe design, delivery, and target accessibility, are also discussed. It is expected that continued advancements in live cell imaging of RNA will open new and exciting opportunities in a wide range of biological and medical applications.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.