ArticlePloS one2009
A single nucleotide change affects fur-dependent regulation of sodB in H. pylori.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 36 citations in OpenAlex.
- Profiling of the Helicobacter pylori redox switch HP1021 regulon using a multi-omics approach.Nature communications · 2023Article
- Divide and conquer: genetics, mechanism, and evolution of the ferrous iron transporter Feo inFrontiers in microbiology · 2023Article
- Hydrogen Peroxide-Mediated Oxygen Enrichment Eradicates Helicobacter pyloriAntimicrobial agents and chemotherapy · 2020Article
- New evolving strategies revealed by transcriptomic analysis of a furMicrobial biotechnology · 2020Article
- ArsRS-Dependent Regulation ofFrontiers in microbiology · 2018Article
- Creation and Initial Characterization of Isogenic Helicobacter pylori CagA EPIYA Variants Reveals Differential Activation of Host Cell Signaling Pathways.Scientific reports · 2017Article
- Characterization of Key Helicobacter pylori Regulators Identifies a Role for ArsRS in Biofilm Formation.Journal of bacteriology · 2016Article
- Helicobacter pylori adaptation in vivo in response to a high-salt diet.Infection and immunity · 2015Article
- The allosteric behavior of Fur mediates oxidative stress signal transduction in Helicobacter pylori.Frontiers in microbiology · 2015Article
- FeON-FeOFF: the Helicobacter pylori Fur regulator commutates iron-responsive transcription by discriminative readout of opposed DNA grooves.Nucleic acids research · 2014Article
- Response to metronidazole and oxidative stress is mediated through homeostatic regulator HsrA (HP1043) in Helicobacter pylori.Journal of bacteriology · 2014Article
- Identification and characterization of novel Helicobacter pylori apo-fur-regulated target genes.Journal of bacteriology · 2013Article
- Random and site-specific mutagenesis of the Helicobacter pylori ferric uptake regulator provides insight into Fur structure-function relationships.Molecular microbiology · 2013Article
- The ferric uptake regulator of Helicobacter pylori: a critical player in the battle for iron and colonization of the stomach.Future microbiology · 2013Review
- High-resolution transcriptome maps reveal strain-specific regulatory features of multiple Campylobacter jejuni isolates.PLoS genetics · 2013Article
- Fur activates expression of the 2-oxoglutarate oxidoreductase genes (oorDABC) in Helicobacter pylori.Journal of bacteriology · 2012Article
- A convenient and robust in vivo reporter system to monitor gene expression in the human pathogen Helicobacter pylori.Applied and environmental microbiology · 2012Article
- Structure and regulon of Campylobacter jejuni ferric uptake regulator Fur define apo-Fur regulation.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Detailed analysis of Helicobacter pylori Fur-regulated promoters reveals a Fur box core sequence and novel Fur-regulated genes.Molecular microbiology · 2012Article
- The oligo-acyl lysyl antimicrobial peptide C₁₂K-2β₁₂ exhibits a dual mechanism of action and demonstrates strong in vivo efficacy against Helicobacter pylori.Antimicrobial agents and chemotherapy · 2012Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Helicobacter pylori is a significant human pathogen that has adapted to survive the many stresses found within the gastric environment. Superoxide Dismutase (SodB) is an important factor that helps H. pylori combat oxidative stress. sodB was previously shown to be repressed by the Ferric Uptake Regulator (Fur) in the absence of iron (apo-Fur regulation) [1]. Herein, we show that apo regulation is not fully conserved among all strains of H. pylori. apo-Fur dependent changes in sodB expression are not observed under iron deplete conditions in H. pylori strains G27, HPAG1, or J99. However, Fur regulation of pfr and amiE occurs as expected. Comparative analysis of the Fur coding sequence between G27 and 26695 revealed a single amino acid difference, which was not responsible for the altered sodB regulation. Comparison of the sodB promoters from G27 and 26695 also revealed a single nucleotide difference within the predicted Fur binding site. Alteration of this nucleotide in G27 to that of 26695 restored apo-Fur dependent sodB regulation, indicating that a single base difference is at least partially responsible for the difference in sodB regulation observed among these H. pylori strains. Fur binding studies revealed that alteration of this single nucleotide in G27 increased the affinity of Fur for the sodB promoter. Additionally, the single base change in G27 enabled the sodB promoter to bind to apo-Fur with affinities similar to the 26695 sodB promoter. Taken together these data indicate that this nucleotide residue is important for direct apo-Fur binding to the sodB promoter.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.