ArticleThe Journal of general physiology2009
A single charged voltage sensor is capable of gating the Shaker K+ channel.
Article in The Journal of general physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Voltage-sensing domains: structural and functional diversity.European biophysics journal : EBJ · 2026Review
- Evaluating sequential and allosteric activation models in IKs channels with mutated voltage sensors.The Journal of general physiology · 2024Article
- Effect of a sensing charge mutation on the deactivation of KV7.2 channels.The Journal of general physiology · 2024Article
- Extracellular protons accelerate hERG channel deactivation by destabilizing voltage sensor relaxation.The Journal of general physiology · 2019Article
- Correction to: Inhibition of the KPflugers Archiv : European journal of physiology · 2018Article
- Inhibition of the KPflugers Archiv : European journal of physiology · 2018Article
- Heat activation is intrinsic to the pore domain of TRPV1.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Inactivation gating of Kv7.1 channels does not involve concerted cooperative subunit interactions.Channels (Austin, Tex.) · 2018Article
- Key role of segment IS4 in Cav1.2 inactivation: link between activation and inactivation.Pflugers Archiv : European journal of physiology · 2017Article
- Mibefradil inhibition of the Cole-Moore shift and KChannels (Austin, Tex.) · 2017Article
- Stabilization of the Activated hERG Channel Voltage Sensor by Depolarization Involves the S4-S5 Linker.Biophysical journal · 2017Article
- Upward movement of IS4 and IIIS4 is a rate-limiting stage in CaPflugers Archiv : European journal of physiology · 2016Article
- Grafting voltage and pharmacological sensitivity in potassium channels.Cell research · 2016Article
- Intersubunit Concerted Cooperative and cis-Type Mechanisms Modulate Allosteric Gating in Two-Pore-Domain Potassium Channel TREK-2.Frontiers in cellular neuroscience · 2016Article
- Alkanols inhibit voltage-gated K(+) channels via a distinct gating modifying mechanism that prevents gate opening.Scientific reports · 2015Article
- A Disease Mutation Causing Episodic Ataxia Type I in the S1 Links Directly to the Voltage Sensor and the Selectivity Filter in Kv Channels.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- The pain receptor TRPV1 displays agonist-dependent activation stoichiometry.Scientific reports · 2015Article
- A distinct sodium channel voltage-sensor locus determines insect selectivity of the spider toxin Dc1a.Nature communications · 2014Article
- Modeling ion channels: past, present, and future.The Journal of general physiology · 2014Review
- Domain IV voltage-sensor movement is both sufficient and rate limiting for fast inactivation in sodium channels.The Journal of general physiology · 2013Article
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2 authors.
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Abstract
We sought to determine the contribution of an individual voltage sensor to Shaker's function. Concatenated heterotetramers of Shaker zH4 Delta(6-46) wild type (wt) in combination with a neutral S4 segment Shaker mutant (mut) with stoichiometries 2wt/2mut and 1wt/3mut were studied and compared with the 4wt concatenated homotetramer. A single charged voltage sensor is sufficient to open Shaker conductance with reduced delay (<1 ms) and at more hyperpolarized voltages compared with 4wt. In addition, the wt-like slow inactivation of 1wt/3mut was almost completely eliminated by mutations T449V-I470C in its single wt subunit, indicating that the subunits bearing a neutral S4 were unable to trigger slow inactivation. Our results strongly suggest that a neutral S4 segment of Shaker's subunit is voltage insensitive and its voltage sensor is in the activated position (i.e., ready for pore opening), and provide experimental support to the proposed model of independent voltage sensors with a final, almost voltage-independent concerted step.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.