ArticleInfection and immunity2009
Purification of Legiobactin and importance of this siderophore in lung infection by Legionella pneumophila.
Article in Infection and immunity, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 47 citations in OpenAlex.
- Major type IV pilin ofmBio · 2026Article
- Modulating backbone flexibility in hydroxamate siderophores for improved iron chelation and peptide nucleic acid delivery into bacteria.The Biochemical journal · 2026Article
- Mucin stimulates the growth ofMicrobiology (Reading, England) · 2026Article
- Article
- Legionella pneumophila Rhizoferrin Promotes Bacterial Biofilm Formation and Growth within Amoebae and Macrophages.Infection and immunity · 2023Article
- Multilocus Sequence Typing for Molecular Epidemiology of Stenotrophomonas maltophilia Clinical and Environmental Isolates from a Tertiary Hospital in West of IranIranian biomedical journal · 2022Article
- Genetic and structural determinants on iron assimilation pathways in the plant pathogen Xanthomonas citri subsp. citri and Xanthomonas sp.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Review
- Assessing the impact, genomics and evolution of type II secretion across a large, medically important genus: the Legionella type II secretion paradigm.Microbial genomics · 2019Article
- Stenotrophomonas maltophilia produces an EntC-dependent catecholate siderophore that is distinct from enterobactin.Microbiology (Reading, England) · 2017Article
- Beyond Paralogs: The Multiple Layers of Redundancy in Bacterial Pathogenesis.Frontiers in cellular and infection microbiology · 2017Review
- Iron Limitation Triggers Early Egress by the Intracellular Bacterial Pathogen Legionella pneumophila.Infection and immunity · 2016Article
- Article
- The Legionella pneumophila Siderophore Legiobactin Is a Polycarboxylate That Is Identical in Structure to Rhizoferrin.Infection and immunity · 2015Article
- MavN is a Legionella pneumophila vacuole-associated protein required for efficient iron acquisition during intracellular growth.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- IroT/mavN, a new iron-regulated gene involved in Legionella pneumophila virulence against amoebae and macrophages.Environmental microbiology · 2015Article
- An update on iron acquisition by Legionella pneumophila: new pathways for siderophore uptake and ferric iron reduction.Future microbiology · 2015Review
- Metabolism of the vacuolar pathogen Legionella and implications for virulence.Frontiers in cellular and infection microbiology · 2014Article
- Secreted pyomelanin of Legionella pneumophila promotes bacterial iron uptake and growth under iron-limiting conditions.Infection and immunity · 2013Article
- Metal ion acquisition in Staphylococcus aureus: overcoming nutritional immunity.Seminars in immunopathology · 2012Review
- The major facilitator superfamily-type protein LbtC promotes the utilization of the legiobactin siderophore by Legionella pneumophila.Microbiology (Reading, England) · 2012Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
When cultured in a low-iron medium, Legionella pneumophila secretes a siderophore (legiobactin) that is both reactive in the chrome azurol S (CAS) assay and capable of stimulating the growth of iron-starved legionellae. Using anion-exchange high-pressure liquid chromatography (HPLC), we purified legiobactin from culture supernatants of a virulent strain of L. pneumophila. In the process, we detected the ferrated form of legiobactin as well as other CAS-reactive substances. Purified legiobactin had a yellow-gold color and absorbed primarily from 220 nm and below. In accordance, nuclear magnetic resonance spectroscopy revealed that legiobactin lacks aromatic carbons, and among the 13 aliphatics present, there were 3 carbonyls. When examined by HPLC, supernatants from L. pneumophila mutants inactivated for lbtA and lbtB completely lacked legiobactin, indicating that the LbtA and LbtB proteins are absolutely required for siderophore activity. Independently derived lbtA mutants, but not a complemented derivative, displayed a reduced ability to infect the lungs of A/J mice after intratracheal inoculation, indicating that legiobactin is required for optimal intrapulmonary survival by L. pneumophila. This defect, however, was not evident when the lbtA mutant and its parental strain were coinoculated into the lung, indicating that legiobactin secreted by the wild type can promote growth of the mutant in trans. Legiobactin mutants grew normally in murine lung macrophages and alveolar epithelial cells, suggesting that legiobactin promotes something other than intracellular infection of resident lung cells. Overall, these data represent the first documentation of a role for siderophore expression in the virulence of L. pneumophila.
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Registered trials
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