ArticleMethods (San Diego, Calif.)2009
Millisecond phase kinetic analysis of elongation catalyzed by human, yeast, and Escherichia coli RNA polymerase.
Article in Methods (San Diego, Calif.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- NTPs compete in the active site of RNA polymerases I and II.Biophysical chemistry · 2024Article
- Reversible Kinetics in Multi-nucleotide Addition Catalyzed by S. cerevisiae RNA polymerase II Reveal Slow Pyrophosphate Release.Journal of molecular biology · 2024Article
- Transient-State Kinetic Analysis of the RNA Polymerase II Nucleotide Incorporation Mechanism.Biochemistry · 2023Article
- An NTP-driven mechanism for the nucleotide addition cycle of Escherichia coli RNA polymerase during transcription.PloS one · 2022Article
- The Role of Pyrophosphorolysis in the Initiation-to-Elongation Transition by E. coli RNA Polymerase.Journal of molecular biology · 2019Article
- Pause sequences facilitate entry into long-lived paused states by reducing RNA polymerase transcription rates.Nature communications · 2018Article
- RNA-DNA and DNA-DNA base-pairing at the upstream edge of the transcription bubble regulate translocation of RNA polymerase and transcription rate.Nucleic acids research · 2018Article
- The A12.2 Subunit Is an Intrinsic Destabilizer of the RNA Polymerase I Elongation Complex.Biophysical journal · 2018Article
- Computational and experimental studies of reassociating RNA/DNA hybrids containing split functionalities.Methods in enzymology · 2015Article
- Molecular basis of transcriptional fidelity and DNA lesion-induced transcriptional mutagenesis.DNA repair · 2014Review
- In silico design and enzymatic synthesis of functional RNA nanoparticles.Accounts of chemical research · 2014Article
- Coliphage HK022 Nun protein inhibits RNA polymerase translocation.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- RNA polymerase structure, function, regulation, dynamics, fidelity, and roles in gene expression.Chemical reviews · 2013Article
- Computational simulation strategies for analysis of multisubunit RNA polymerases.Chemical reviews · 2013Review
- Intrinsic translocation barrier as an initial step in pausing by RNA polymerase II.Journal of molecular biology · 2013Article
- The RNA polymerase bridge helix YFI motif in catalysis, fidelity and translocation.Biochimica et biophysica acta · 2013Article
- Isolation and characterization of RNA polymerase rpoB mutations that alter transcription slippage during elongation in Escherichia coli.The Journal of biological chemistry · 2013Article
- Molecular dynamics and mutational analysis of the catalytic and translocation cycle of RNA polymerase.BMC biophysics · 2012Article
- Dissecting chemical interactions governing RNA polymerase II transcriptional fidelity.Journal of the American Chemical Society · 2012Article
- Sensitivity of mitochondrial transcription and resistance of RNA polymerase II dependent nuclear transcription to antiviral ribonucleosides.PLoS pathogens · 2012Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Strategies for assembly and analysis of human, yeast, and bacterial RNA polymerase elongation complexes are described, and methods are shown for millisecond phase kinetic analyses of elongation using rapid chemical quench flow. Human, yeast, and bacterial RNA polymerases function very similarly in NTP-Mg2+ commitment and phosphodiester bond formation. A "running start, two-bond, double-quench" protocol is described and its advantages discussed. These studies provide information about stable NTP-Mg2+ loading, phosphodiester bond synthesis, the processive transition between bonds, and sequence-specific effects on transcription elongation dynamics.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.