ArticleMethods (San Diego, Calif.)2009
Targeted gene disruption in Francisella tularensis by group II introns.
Article in Methods (San Diego, Calif.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Mobilizable shuttle vectors with fluorescent markers functional across different species of bacteria.Applied and environmental microbiology · 2025Article
- A study of innate immune kinetics reveals a role for a chloride transporter in a virulent Francisella tularensis type B strain.MicrobiologyOpen · 2021Article
- Consolidated bioprocessing for butanol production of cellulolytic Clostridia: development and optimization.Microbial biotechnology · 2020Review
- Article
- The Multiple Localized Glyceraldehyde-3-Phosphate Dehydrogenase Contributes to the Attenuation of theFrontiers in cellular and infection microbiology · 2017Article
- Metabolic Engineering of Raoultella ornithinolytica BF60 for Production of 2,5-Furandicarboxylic Acid from 5-Hydroxymethylfurfural.Applied and environmental microbiology · 2017Article
- Generating Isogenic Deletions (Knockouts) inBio-protocol · 2015Article
- Characterization of tetratricopeptide repeat-like proteins in Francisella tularensis and identification of a novel locus required for virulence.Infection and immunity · 2014Article
- Disruption of Francisella tularensis Schu S4 iglI, iglJ, and pdpC genes results in attenuation for growth in human macrophages and in vivo virulence in mice and reveals a unique phenotype for pdpC.Infection and immunity · 2013Article
- Site-specific, insertional inactivation of incA in Chlamydia trachomatis using a group II intron.PloS one · 2013Article
- Generalized bacterial genome editing using mobile group II introns and Cre-lox.Molecular systems biology · 2013Article
- Francisella tularensis tmRNA system mutants are vulnerable to stress, avirulent in mice, and provide effective immune protection.Molecular microbiology · 2012Article
- Identification and characterization of novel and potent transcription promoters of Francisella tularensis.Applied and environmental microbiology · 2011Article
- Group II intron-anchored gene deletion in Clostridium.PloS one · 2011Article
- Macrophage replication screen identifies a novel Francisella hydroperoxide resistance protein involved in virulence.PloS one · 2011Article
- Genetic manipulation of francisella tularensis.Frontiers in microbiology · 2010Article
- Utilization of an unstable plasmid and the I-SceI endonuclease to generate routine markerless deletion mutants in Francisella tularensis.Journal of microbiological methods · 2010Article
- Working toward the future: insights into Francisella tularensis pathogenesis and vaccine development.Microbiology and molecular biology reviews : MMBR · 2009Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Francisella tularensis is a highly infectious Gram-negative bacterium that is the causative agent of tularemia. Very little is known about the molecular mechanisms responsible for F. tularensis virulence, in part due to the paucity of genetic tools available for the study of F. tularensis. We have developed a gene knockout system for F. tularensis that utilizes retargeted mobile group II introns, or "targetrons". These targetrons disrupt both single and duplicated target genes at high efficiency in three different F. tularensis subspecies. Here we describe in detail the targetron-based method for insertional mutagenesis of F. tularensis genes, which should facilitate a better understanding of F. tularensis pathogenesis. Group II introns can be adapted to inactivate genes in bacteria for which few genetic tools exist, thus providing a powerful tool to study the genetic basis of bacterial pathogenesis.
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