ArticleJournal of experimental & clinical cancer research : CR2009
Flow cytometric analysis of CK19 expression in the peripheral blood of breast carcinoma patients: relevance for circulating tumor cell detection.
Article in Journal of experimental & clinical cancer research : CR, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- The impact of liquid biopsy in breast cancer: Redefining the landscape of non-invasive precision oncology.The journal of liquid biopsy · 2025Review
- Isolating Circulating Cancer Stem Cells (CCSCs) from Human Whole Blood.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Quantitative CK19 biomarker detection in breast cancer cell lines.Journal of medicine and life · 2022Article
- Clinical significance of cytokeratin 19 and OCT4 as survival markers in non-metastatic and metastatic breast cancer patients.Contemporary oncology (Poznan, Poland) · 2022Article
- Enrichment and detection of circulating tumor cells by immunomagnetic beads and flow cytometry.Biotechnology letters · 2021Article
- Less micrometastatic risk related to circulating tumor cells after endoscopic breast cancer surgery compared to open surgery.BMC cancer · 2019Observational
- EpCAM-based Flow Cytometric Detection of Circulating Tumor Cells in Gallbladder Carcinoma CasesAsian Pacific journal of cancer prevention : APJCP · 2017Article
- Expression and Clinical Significance of Cytokeratin-19 and Thymidine Kinase-1 in Advanced Gastrointestinal Cancer.Chinese medical journal · 2016Article
- Notes for developing a molecular test for the full characterization of circulating tumor cells.Chinese journal of cancer research = Chung-kuo yen cheng yen chiu · 2015Review
- Methods for detecting circulating cancer stem cells (CCSCs) as a novel approach for diagnosis of colon cancer relapse/metastasis.Laboratory investigation; a journal of technical methods and pathology · 2015Article
- Article
- Clinical application of circulating tumor cells in breast cancer.Cellular oncology (Dordrecht, Netherlands) · 2014Review
- [Advances in research on circulating tumor cells in lung cancer].Zhongguo fei ai za zhi = Chinese journal of lung cancer · 2012Review
- Isolated, disseminated and circulating tumour cells in prostate cancer.Nature reviews. Urology · 2012Review
- Clinical significance of stanniocalcin-1 detected in peripheral blood and bone marrow of esophageal squamous cell carcinoma patients.Journal of experimental & clinical cancer research : CR · 2012Article
- Detection of circulating tumor cells and tumor stem cells in patients with breast cancer by using flow cytometry: a valuable tool for diagnosis and prognosis evaluation.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2012Article
- Fluorescent metal nanoshell and CK19 detection on single cell image.Biochemical and biophysical research communications · 2011Article
- Circulating tumor cells: advances in detection methods, biological issues, and clinical relevance.Journal of cancer research and clinical oncology · 2011Review
- Circulating tumor cells in breast cancer patients: an evolving role in patient prognosis and disease progression.Pathology research international · 2011Article
- Gene expression of circulating tumour cells and its correlation with tumour stage in breast cancer patients.European journal of medical research · 2009Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundImmunocytochemistry and RT-PCR have been widely used for the detection of circulating tumor cells in patients with breast cancer but their specificity is limited. Our purpose is to utilize a convenient and specific technology to detect circulating tumor cells in breast cancer patients.
methodsTo determine the sensitivity and specificity of our method, A431 cells were serially diluted with human peripheral blood leukocytes and stained with CK19. A total of 73 blood specimens including 25 healthy volunteers and 48 patients with breast carcinoma and benign tumor were tested by flow cytometry to quantify the expression of CK19.
resultsThe detectable upper limit of A431 cells was 1 cancer cell among 104 human white blood cells. CK19 was detected in 27% of breast cancer patients but none control gives positive result. The number of cancer cells increased gradually along with the disease stages for it was the least in stage I (0%) and the most in stage IV (1.29%). Fifteen patients were observed during three month chemotherapy after surgery, and most of their CK19 expression levels declined after treatment.
conclusionOur research convinces that the detection of CK19 in peripheral blood by flow cytometry is also a specific and feasible method to monitor circulating tumor cells in breast cancer.
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