ArticleJournal of cellular and molecular medicine2009
Laminin isoforms in human embryonic stem cells: synthesis, receptor usage and growth support.
Article in Journal of cellular and molecular medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 50 citations in OpenAlex.
- Integrin signaling in pluripotent cells acts as a gatekeeper of mouse germline entry.Science advances · 2024Article
- Differentiating Human Pluripotent Stem Cells to Cardiomyocytes Using Purified Extracellular Matrix Proteins.Bioengineering (Basel, Switzerland) · 2022Review
- Human Induced Pluripotent Stem Cell (iPSC) Handling Protocols: Maintenance, Expansion, and Cryopreservation.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Discovery of a Novel Polymer for Xeno-Free, Long-Term Culture of Human Pluripotent Stem Cell Expansion.Advanced healthcare materials · 2021Article
- Gene Expression in Amnion-Derived Cells Cultured on Recombinant Laminin 332-A Preliminary Study.Frontiers in medicine · 2021Article
- The role of integrin β1 in the heterogeneity of human embryonic stem cells culture.Biology open · 2018Article
- The role of laminins in cartilaginous tissues: from development to regeneration.European cells & materials · 2017Review
- A Strong Contractile Actin Fence and Large Adhesions Direct Human Pluripotent Colony Morphology and Adhesion.Stem cell reports · 2017Article
- Biological Effects of Culture Substrates on Human Pluripotent Stem Cells.Stem cells international · 2016Review
- Cultured Cells from the Human Oocyte Cumulus Niche Are Efficient Feeders to Propagate Pluripotent Stem Cells.Stem cells and development · 2015Article
- Discovery of a Novel Polymer for Human Pluripotent Stem Cell Expansion and Multilineage Differentiation.Advanced materials (Deerfield Beach, Fla.) · 2015Article
- Changes in Laminin Expression Pattern during Early Differentiation of Human Embryonic Stem Cells.PloS one · 2015Article
- Differentiation of human embryonic stem cells to hepatocyte-like cells on a new developed xeno-free extracellular matrix.Histochemistry and cell biology · 2014Article
- CCL2 enhances pluripotency of human induced pluripotent stem cells by activating hypoxia related genes.Scientific reports · 2014Article
- Development of a simple, repeatable, and cost-effective extracellular matrix for long-term xeno-free and feeder-free self-renewal of human pluripotent stem cells.Histochemistry and cell biology · 2013Article
- A novel feeder-free culture system for human pluripotent stem cell culture and induced pluripotent stem cell derivation.PloS one · 2013Article
- MUC1* ligand, NM23-H1, is a novel growth factor that maintains human stem cells in a more naïve state.PloS one · 2013Article
- Comparison of several attachment methods for human iPS, embryonic and adipose-derived stem cells for tissue engineering.Journal of tissue engineering and regenerative medicine · 2012Article
- A synthetic, xeno-free peptide surface for expansion and directed differentiation of human induced pluripotent stem cells.PloS one · 2012Article
- Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells.Nature communications · 2012Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To reveal the functional intrinsic niche of human embryonic stem cells (hESC) we examined the production of basement membrane (BM) proteins and the presence of their receptors in feeder-free cell culture conditions. In addition, we investigated binding of hESCs to purified human BM proteins and identified the receptors mediating these contacts. Also, we tested whether purified human laminin (Lm) isoforms have a role in hESC self-renewal and growth in short-term cultures. The results show that hESCs synthesize Lm alpha(1) and Lm alpha(5) chains together with Lm beta(1) and gamma(1) chains suggesting the production of Lms-111 and -511 into the culture medium and deposits on cells. hESCs contain functionally important integrin (Int) subunits, Int beta(1), alpha(3), alpha(6), alpha(5), beta(5) and alpha(V), as well as the Lm alpha(5) receptor, Lutheran (Lu) glycoprotein and its truncated form, basal cell adhesion molecule (B-CAM). In cell adhesion experiments, Int beta(1) was crucial for adhesion to most of the purified human BM proteins. Lu/B-CAM mediated adhesion to Lm-511 together with Int alpha(3)beta(1), and was essential for the adhesion of hESCs to embryonic feeder cells. Adhesion to Lm-411 was mediated by Int alpha(6)beta(1). Lm-511 supported hESC growth in defined medium equally well as Matrigel. These results provide consequential information of the biological role of BM in hESCs, warranting further investigation of BM biology of human pluripotent stem cells.
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