ArticleCell proliferation2009
In vitro three-dimensional modelling of human ovarian surface epithelial cells.
Article in Cell proliferation, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.
- Common variants at the CHEK2 gene locus and risk of epithelial ovarian cancer.Carcinogenesis · 2015Pooled it
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- Effects of long-term norepinephrine treatment on normal immortalized ovarian and fallopian tube cells.Scientific reports · 2021Article
- Organoid systems to study the human female reproductive tract and pregnancy.Cell death and differentiation · 2021Review
- Human Female Reproductive System Organoids: Applications in Developmental Biology, Disease Modelling, and Drug Discovery.Stem cell reviews and reports · 2020Review
- A transcriptome-wide association study of high-grade serous epithelial ovarian cancer identifies new susceptibility genes and splice variants.Nature genetics · 2019Article
- Functional Analysis and Fine Mapping of the 9p22.2 Ovarian Cancer Susceptibility Locus.Cancer research · 2019Article
- Review
- Early transcriptional response of human ovarian and fallopian tube surface epithelial cells to norepinephrine.Scientific reports · 2018Article
- High-throughput 3-dimensional culture of epithelial ovarian cancer cells as preclinical model of disease.Oncotarget · 2018Article
- Article
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- Ovary and fimbrial stem cells: biology, niche and cancer origins.Nature reviews. Molecular cell biology · 2015Review
- Stromal Expression of Fibroblast Activation Protein Alpha (FAP) Predicts Platinum Resistance and Shorter Recurrence in patients with Epithelial Ovarian Cancer.Cancer microenvironment : official journal of the International Cancer Microenvironment Society · 2015Article
- Novel three-dimensional in vitro models of ovarian endometriosis.Journal of ovarian research · 2014Article
- Modeling platinum sensitive and resistant high-grade serous ovarian cancer: development and applications of experimental systems.Frontiers in oncology · 2014Review
- Induction of estrogen-sensitive epithelial cells derived from human-induced pluripotent stem cells to repair ovarian function in a chemotherapy-induced mouse model of premature ovarian failure.DNA and cell biology · 2013Article
- Lessons from postgenome-wide association studies: functional analysis of cancer predisposition loci.Journal of internal medicine · 2013Review
- In vitro three-dimensional modeling of fallopian tube secretory epithelial cells.BMC cell biology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
objectivesNinety percent of malignant ovarian cancers are epithelial and thought to arise from the ovarian surface epithelium (OSE). We hypothesized that biological characteristics of primary OSE cells would more closely resemble OSE in vivo if established as three-dimensional (3D) cultures. MATERIALS AND
methodsOSE cells were cultured as multicellular spheroids (MCS) (i) in a rotary cell culture system (RCCS) and (ii) on polyHEMA-coated plastics. The MCSs were examined by electron microscopy and compared to OSE from primary tissues and cells grown in 2D. Annexin V FACS analysis was used to evaluate apoptosis and expression of extracellular matrix (ECM) proteins was analysed by immunohistochemical staining.
resultsOn polyHEMA-coated plates, OSE spheroids had defined internal architecture. RCCS MCSs had disorganized structure and higher proportion of apoptotic cells than polyHEMA MCSs and the same cells grown in 2D culture. In 2D, widespread expression of AE1/AE3, laminin and vimentin were undetectable by immunohistochemistry, whereas strong expression of these proteins was observed in the same cells grown in 3D culture and in OSE on primary tissues.
conclusionsPhysiological and biological features of OSE cells grown in 3D culture more closely resemble characteristics of OSE cells in vivo than when grown by classical 2D approaches. It is likely that establishing in vitro 3D OSE models will lead to greater understanding of the mechanisms of neoplastic transformation in epithelial ovarian cancers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.