ArticleLangmuir : the ACS journal of surfaces and colloids2009
Comprehensive analysis of lipid dynamics variation with lipid composition and hydration of bicelles using nuclear magnetic resonance (NMR) spectroscopy.
Article in Langmuir : the ACS journal of surfaces and colloids, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 54 citations in OpenAlex.
- Solid-State NMR of Membrane Proteins in Lipid Bilayers: To Spin or Not To Spin?Accounts of chemical research · 2021Review
- Styrene maleic acid derivates to enhance the applications of bio-inspired polymer based lipid-nanodiscs.European polymer journal · 2018Article
- Probing Residue-Specific Water-Protein Interactions in Oriented Lipid Membranes via Solid-State NMR Spectroscopy.The journal of physical chemistry. B · 2016Article
- Lipid concentration and molar ratio boundaries for the use of isotropic bicelles.Langmuir : the ACS journal of surfaces and colloids · 2014Article
- Article
- Membrane disordering is not sufficient for membrane permeabilization by islet amyloid polypeptide: studies of IAPP(20-29) fragments.Physical chemistry chemical physics : PCCP · 2013Article
- When detergent meets bilayer: birth and coming of age of lipid bicelles.Progress in nuclear magnetic resonance spectroscopy · 2013Review
- The magic of bicelles lights up membrane protein structure.Chemical reviews · 2012Review
- Fast NMR data acquisition from bicelles containing a membrane-associated peptide at natural-abundance.The journal of physical chemistry. B · 2011Article
- A proton spin diffusion based solid-state NMR approach for structural studies on aligned samples.The journal of physical chemistry. B · 2011Article
- Chemical shift tensor - the heart of NMR: Insights into biological aspects of proteins.Progress in nuclear magnetic resonance spectroscopy · 2010Review
- Orientation, dynamics, and lipid interaction of an antimicrobial arylamide investigated by 19F and 31P solid-state NMR spectroscopy.Journal of the American Chemical Society · 2010Article
- Solid-state NMR reveals the hydrophobic-core location of poly(amidoamine) dendrimers in biomembranes.Journal of the American Chemical Society · 2010Article
- Use of a copper-chelated lipid speeds up NMR measurements from membrane proteins.Journal of the American Chemical Society · 2010Article
- Proton-evolved local-field solid-state NMR studies of cytochrome b5 embedded in bicelles, revealing both structural and dynamical information.Journal of the American Chemical Society · 2010Article
- Assessing the size, stability, and utility of isotropically tumbling bicelle systems for structural biology.Biochimica et biophysica acta · 2010Article
- Anesthetic effects on the structure and dynamics of the second transmembrane domains of nAChR alpha4beta2.Biochimica et biophysica acta · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Bicelles of various lipid/detergent ratios are commonly used in nuclear magnetic resonance (NMR) studies of membrane-associated molecules without the need to freeze the sample. While a decrease in the size (defined at a low temperature or by the q value) of a bicelle decreases its overall order parameter, the variation of lipid dynamics with a change in the lipid/detergent ratio is unknown. In this study, we report a thorough atomistic level analysis on the variation of lipid dynamics with the size and hydration level of bicelles composed of a phospholipid, 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), and a detergent, 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC). Two-dimensional (2D) separated-local-field NMR experiments were performed on magnetically aligned bicelles to measure (1)H-(13)C dipolar couplings, which were used to determine order parameters at various (head-group, glycerol, and acyl chain) regions of lipids in the bilayer. From our analysis, we uncover the extreme sensitivity of the glycerol region to the motion of the bicelle, which can be attributed to the effect of viscosity because of an extensive network of hydrogen bonds. As such, the water-membrane interface region exhibits the highest order parameter values among all three regions of a lipid molecule. Our experimental results demonstrate that the laboratory-frame 2D proton-detected-local-field pulse sequence is well-suited for the accurate measurement of motionally averaged (or long-range) weak and multiple (1)H-(13)C dipolar couplings associated with a single carbon site at the natural abundance of (13)C nuclei.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.