ReviewCurrent opinion in drug discovery & development2009
Pooling in high-throughput drug screening.
Review in Current opinion in drug discovery & development, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed.
- A Scalable Flow Cytometry Assay to Evaluate CatSper-mediated CaAndrology · 2026Article
- Combinatorial group testing for efficient scaling across biological applications.Nature communications · 2026Article
- Article
- Scalable, compressed phenotypic screening using pooled perturbations.Nature biotechnology · 2025Article
- Uncovering Novel Drugs that Restore Vision Using Orthogonal Pooling in Zebrafish.Advances in experimental medicine and biology · 2025Article
- MultiplexMS: A Mass Spectrometry-Based Multiplexing Strategy for Ultra-High-Throughput Analysis of Complex Mixtures.Analytical chemistry · 2023Article
- Evaluation of a Pooling Chemoproteomics Strategy with an FDA-Approved Drug Library.Biochemistry · 2023Article
- Capturing the pool dilution effect in group testing regression: A Bayesian approach.Statistics in medicine · 2022Article
- Synergizing the potential of bacterial genomics and metabolomics to find novel antibiotics.Chemical science · 2021Review
- Phenotypic Screening inPharmaceuticals (Basel, Switzerland) · 2020Review
- Creating and screening natural product libraries.Natural product reports · 2020Review
- When to use one-dimensional, two-dimensional, and Shifted Transversal Design pooling in mycotoxin screening.PloS one · 2020Article
- The objective function controversy for group testing: Much ado about nothing?Statistics in medicine · 2019Article
- Informative group testing for multiplex assays.Biometrics · 2019Article
- Orthogonal Drug Pooling Enhances Phenotype-Based Discovery of Ocular Antiangiogenic Drugs in Zebrafish Larvae.Frontiers in pharmacology · 2019Review
- Toward a Microparticle-Based System for Pooled Assays of Small Molecules in Cellular Contexts.ACS chemical biology · 2018Article
- Identification of sirtuin 5 inhibitors by ultrafast microchip electrophoresis using nanoliter volume samples.Analytical and bioanalytical chemistry · 2016Article
- Designing innovative therapies for neuropathic pain: pros and cons of target-based drug discovery.Journal of the peripheral nervous system : JPNS · 2014Article
- An inverse small molecule screen to design a chemically defined medium supporting long-term growth of Drosophila cell lines.Molecular bioSystems · 2014Article
- Drug screening strategy for human membrane proteins: from NMR protein backbone structure to in silica- and NMR-screened hits.Biochemical and biophysical research communications · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Pooling in HTS refers to the act of testing mixtures of compounds in a primary screen to accurately identify hits for secondary screening. The reduction in the number of tests needed to screen a compound library by pooling can also be extended to achieve much-needed error tolerance in HTS. Despite the success of HTS in other biological experiments, pooling in high-throughput drug screening has been a controversial and often marginalized paradigm. At first appearance, pooling appears to promise gains from reduced effort, or possibly could create more problems than solutions. However, this article demonstrates that pooling is a practical and necessary part of HTS: discussions include the rationale for pooling compounds in HTS, a unifying view of pooling design theory, a review of past attempts at pooling and their success, and recent advances in the field.
Indexed as
Identifiers
19396735PMC3204799What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.