ArticleJournal of visualized experiments : JoVE2009
Measuring near plasma membrane and global intracellular calcium dynamics in astrocytes.
Article in Journal of visualized experiments : JoVE, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 16 citations in OpenAlex.
- Homer1 Scaffold Proteins Govern Ca2+ Dynamics in Normal and Reactive Astrocytes.Cerebral cortex (New York, N.Y. : 1991) · 2017Article
- Imaging intracellular Ca²⁺ signals in striatal astrocytes from adult mice using genetically-encoded calcium indicators.Journal of visualized experiments : JoVE · 2014Article
- Imaging P2X4 receptor subcellular distribution, trafficking, and regulation using P2X4-pHluorin.The Journal of general physiology · 2014Article
- TRPA1 channels are regulators of astrocyte basal calcium levels and long-term potentiation via constitutive D-serine release.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Inflammatory mediators alter the astrocyte transcriptome and calcium signaling elicited by multiple G-protein-coupled receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- TRPA1 channels regulate astrocyte resting calcium and inhibitory synapse efficacy through GAT-3.Nature neuroscience · 2011Article
- Neuronal P2X2 receptors are mobile ATP sensors that explore the plasma membrane when activated.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Somatic ATP release from guinea pig sympathetic neurons does not require calcium-induced calcium release from internal stores.American journal of physiology. Cell physiology · 2010Article
- Monitoring astrocyte calcium microdomains with improved membrane targeted GCaMP reporters.Neuron glia biology · 2010Article
- A genetically targeted optical sensor to monitor calcium signals in astrocyte processes.Nature neuroscience · 2010Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The brain contains glial cells. Astrocytes, a type of glial cell, have long been known to provide a passive supportive role to neurons. However, increasing evidence suggests that astrocytes may also actively participate in brain function through functional interactions with neurons. However, many fundamental aspects of astrocyte biology remain controversial, unclear and/or experimentally unexplored. One important issue is the dynamics of intracellular calcium transients in astrocytes. This is relevant because calcium is well established as an important second messenger and because it has been proposed that astrocyte calcium elevations can trigger the release of transmitters from astrocytes. However, there has not been any detailed or satisfying description of near plasma membrane calcium signaling in astrocytes. Total internal reflection fluorescence (TIRF) microscopy is a powerful tool to analyze physiologically relevant signaling events within about 100 nm of the plasma membrane of live cells. Here, we use TIRF microscopy and describe how to monitor near plasma membrane and global intracellular calcium dynamics almost simultaneously. The further refinement and systematic application of this approach has the potential to inform about the precise details of astrocyte calcium signaling. A detailed understanding of astrocyte calcium dynamics may provide a basis to understand if, how, when and why astrocytes and neurons undergo calcium-dependent functional interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.